Non-transgenic Herbicide-resistant Cytoplasmic Male Sterile Lines of Brassica napus L.: Breeding and Application

doi:10.11924/j.issn.1000-6850.casb20200100017

Abstract

Abstract:

The aims are to breed herbicide-resistant rapeseed varieties suitable for planting in China, improve the efficiency of field chemical weeding and reduce the cost of rapeseed production. ‘Shan 9B’ was treated with 0.5% EMS solution to create germplasm resources with herbicide resistance. By a large quantity of screening, a rape strain ‘Shan 9BK’ with herbicide resistance was obtained, and then it was used as the recurrent parent to breed rape cytoplasmic male sterile line ‘Shan 9AK ’with herbicide resistance; ‘Shan 9AK’ was used as female parent to make hybrid combinations, the combinations of ‘Shan 9AK×R3’ ‘Shan 9AK×R6’ and ‘Shan 9AK×R1’ had stronger growth vigor, uniformity, high yield, yield-increasing potential, disease resistance and lodging resistance. They also had strong resistance to herbicide at the seedling stage, and were suitable for chemical weeding in field. Cytoplasmic male sterile line ‘Shan 9AK’ is beneficial to breed herbicide-resistant hybrid combinations, which can simplify the seed production procedure of rapeseed in hybrid seed production, reduce seed production cost and improve farmers’ planting efficiency.

Key words: non-transgenic, herbicide resistance, Brassica napus L., cytoplasmic male sterile line, variety breeding

CLC Number:

S565.4

Guan Zhoubo, Dong Yuhong, Tian Jianhua, Wang Xuefang, Zhang Zhongxin, Zheng Lei. Non-transgenic Herbicide-resistant Cytoplasmic Male Sterile Lines of Brassica napus L.: Breeding and Application[J]. Chinese Agricultural Science Bulletin, 2020, 36(26): 9-13.

Figures/Tables 4

References 29

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组合编号	小区产量/kg				产量/(kg/hm²)	比CK/±%	位次
组合编号	Ⅰ	Ⅱ	Ⅲ	平均	产量/(kg/hm²)	比CK/±%	位次
陕9AK×R1	6.86	6.59	6.33	6.59	3298.32	4.88%	3
陕9AK×R2	6.30	6.44	6.58	6.44	3221.61	2.44%	5
陕9AK×R3	6.55	6.67	6.95	6.72	3363.35	6.95%	1
陕9AK×R4	6.62	6.48	6.33	6.48	3239.95	3.02%	4
秦优7号(CK)	6.22	6.31	6.33	6.29	3144.91	0.00%	6
陕9AK×R5	6.24	6.33	6.18	6.25	3126.56	-0.58%	7
陕9AK×R6	6.64	6.58	6.73	6.65	3326.66	5.78%	2
陕9AK×R7	6.38	6.12	6.24	6.25	3124.90	-0.64%	8

组合编号	小区产量/kg				产量/(kg/hm²)	比CK/±%	位次
组合编号	Ⅰ	Ⅱ	Ⅲ	平均	产量/(kg/hm²)	比CK/±%	位次
陕9AK×R1	6.86	6.59	6.33	6.59	3298.32	4.88%	3
陕9AK×R2	6.30	6.44	6.58	6.44	3221.61	2.44%	5
陕9AK×R3	6.55	6.67	6.95	6.72	3363.35	6.95%	1
陕9AK×R4	6.62	6.48	6.33	6.48	3239.95	3.02%	4
秦优7号(CK)	6.22	6.31	6.33	6.29	3144.91	0.00%	6
陕9AK×R5	6.24	6.33	6.18	6.25	3126.56	-0.58%	7
陕9AK×R6	6.64	6.58	6.73	6.65	3326.66	5.78%	2
陕9AK×R7	6.38	6.12	6.24	6.25	3124.90	-0.64%	8