Resistance Monitoring of Alopecurus aequalis Sobol. to Quizalofop-P-ethyl and Cross-resistance with Clethodim in Rape Field

doi:10.11924/j.issn.1000-6850.casb15120093

Abstract

Abstract: By detecting the resistance level of Alopecurus aequalis Sobol., and studying its resistance mechanism, a theoretical basis for rational drug use can be provided for A.aequalis resistance management in rape field. The resistance levels of Alopecurus aequalis Sobol. biotypes to quizalofop-P-ethyl and the cross-resistance with clethodim in 17 areas in the middle and lower reaches of Yangtze River were determined using the whole-plant assay method. The glutathione-s-transferase (GST_S) enzyme activity was studied in resistant biotype (R-TY) and susceptible biotype (S-NJ) with quizalofop-P-ethyl and clethodim. The anti-quizalofop monitoring results showed that: TY biotype had the highest relative resistance multiple, which was 28.92, GR₅₀ was 90.183. And then were YZ and BLD biotype, of which, GR₅₀ was 34.480 and 45.472, respectively, and the relative resistance multiple was 11.06 and 14.58, respectively. The relative resistance multiples of other biotypes were ranged from 1.62 to 9.16. The cross-resistance with clethodim showed that there was a cross-resistance between quizalofop and clethodim in TY and BLD biotype, while DYX, the quizalofop sensitive biotype, was resistant to clethodim, indicating A. aequalis biotypes might have other resistance patterns. GST_S activity assay showed that: after drug treatment, the GST_S activity in R-TY biotype was always higher than that in the sensitive biotype S-NJ, indicating that TY biotype’s resistance to drug might be due to the GST_S enhancing effect on drug metabolism.

Tang Zihui and Zhou Xiaomao. Resistance Monitoring of Alopecurus aequalis Sobol. to Quizalofop-P-ethyl and Cross-resistance with Clethodim in Rape Field[J]. Chinese Agricultural Science Bulletin, 2016, 32(18): 170-175.

References

[24] 祝玮玮,刘艺妩,刘祥英,等.油菜田看麦娘对精喹禾灵的抗性水平及抗性机理研究[J].植物保护,2014,40(5):80-85.
[25] Herbicide Resistance Action Committee [EB/OL]. [2013-08-03]. http://www.hracglobal.com.
[26] 马晓渊.农田杂草抗药性的发生为害、原因与治理[J].杂草科学,2002(1):5-9.
[27] Devine M D. Mechanisms of resistance to acetyl-coenzyme A carboxylase inhibitors: a review [J]. Pesticide Science, 1997,51(3):259-264.
[28] Christophye D. Weed resistance to Acetyl-coenzyme A carboxylase inhibitors: an update [J]. Weed Science, 2005, 53: 728-746.
[29] Cummins I, Wortley D J, Sabbadin F, et al. Key role for a glutathione transferase in multiple-herbicide resistance in grass weeds[J]. Proceedings of the National Academy of Sciences, 2013, 110(15): 5812-5817.
[30] Cummins I, Moss S, Cole D J, et al. Glutathione transferases in herbicide‐resistant and herbicide‐susceptible black-grass (Alopecurus myosuroides)[J]. Pesticide science, 1997, 51(3):244-250.[1] 王汉中,官春云,张春雷.中国的油菜生产与栽培科学技术研究[A].第十二届国际油菜大会论文集[C].2007:2-7.
[2] 苏少泉,宋顺祖.中国农田杂草化学防治[M].北京:中国农业出版社,1996.
[3] 孙炳耀.麦田看麦娘属杂草种群消长原因及其抗药性鉴定的研究[D]. 南京:南京农业大学,1996,
[4] 赵玉信,杨惠敏.物格局、土壤耕作和水肥管理对农田杂草发生的影响及其调控机制[J].草业学报,2015,24(8):199-210.
[5] 韩敏,李粉华,孙国俊,等.江苏金坛地区小麦田杂草种群生态位研究[J].江西农业学报,2015,27(9):45-49.
[6] 鲁军雄,陈社员,官春云,等.长江中下游油菜田杂草发生规律及实用防治策略[J].作物研究,2012,26(6):639-642.
[7] 赵虎基,王国英.植物乙酷辅酶A羧化酶的分子生物学与基因工程[J].中国生物工程杂志,2003,23(2):12-16.
[8] 黄世霞,王庆亚,董立尧,等.乙酰辅酶Ａ羧化酶抑制剂类除草剂与杂草的抗药性[J].杂草科学,2003(2):1-5.
[9] Zhang X Q, Powles B S. The Molecular Bases for Resistance to Acetyl-coenzyme A Carboxylase (ACCase) Inhibiting Herbicides in Two Target-based Resistant Biotypes of Annual Ryegrass(Lolium rigidum)[J]. Planta, 2006, 223: 550-557.
[10] Marles M A S, Devine M D, Hall J C. Herbicide resistance in Setaria viridis conferred by a less sensitive form of acetyl coenzyme A carboxylase[J]. Pesticide Biochemistry and Physiology, 1993, 46(1): 7-14.
[11] Devine M D. Mechanisms of resistance to acetyl-coenzyme A carboxylase inhibitors: a review[J]. Pesticide Science, 1997, 51(3): 259-264.
[12] Moss S R, Cussans G W. The development of herbicide resistant populations of Alopecurus myosuroides (black-grass) in England[J]. Herbicide resistance in weeds and crops, 1991: 45-55.
[13] 黄世霞,王庆亚,张守栋.油菜田看麦娘对精喹禾灵和烯禾啶交互抗性[J].农药,2008,47(9):679-681.
[14] Guo W, Liu W, Li L, et al. Molecular Basis for Resistance to Fenoxaprop in Shortawn Foxtail (Alopecurus aequalis) from China[J]. Weed Science, 2015, 63(2): 416-424.
[15] Marrs K A. The functions and regulation of glutathione S-transferases in plants[J]. Annual review of plant biology, 1996, 47(1): 127-158.
[16] Neuefeind T, Reinemer P, Bieseler B. Plant glutathione S-transferases and herbicide detoxification[J]. Biological chemistry, 1996, 378(3-4): 199-205.
[17] Timmerman K P. Molecular characterization of corn glutathione S-transferase isozymes involved in herbicide detoxication[J]. Physiologia Plantarum, 1989, 77(3): 465-471.
[18] Hall L M, Moss S R, Powles S B. Mechanisms of resistance to aryloxyphenoxypropionate herbicides in two resistance biotypes of Alopecurus mysouroides (black grass): herbicide metabolism as a cross-resistance mechanism [J]. Pesticide Biochemistry and Physiology, 1997,57(2):87-98.
[19] Czarnota M A, Rimando A M, Weston L A. Evaluation of root exudates of seven sorghum accessions. Journal of Chemical Ecology,2003, 29(9):2073-2083.
[20] 王庆亚,董立尧,娄远来,等.田杂草抗药性及其检测鉴定方法[J].杂草科学,2002(2):1-5.
[21] 张朝贤,倪汉文,魏守辉,等.杂草抗药性研究进展[J].中国农业科学,2009,42(4):1274-1289.
[22] 吴进才,徐俊峰,冯绪猛,等.稻田常用农药对水稻3个品种生理生化的影响[J].中国农业科学,2003,36(5):536-541.
[23] 韩瑞娟.日本看麦娘对高效氟吡甲禾灵抗性机理的研究[D].南京:南京农业大学,2010.
[24] 祝玮玮,刘艺妩,刘祥英,等.油菜田看麦娘对精喹禾灵的抗性水平及抗性机理研究[J].植物保护,2014,40(5):80-85.
[25] Herbicide Resistance Action Committee [EB/OL]. [2013-08-03]. http://www.hracglobal.com.
[26] 马晓渊.农田杂草抗药性的发生为害、原因与治理[J].杂草科学,2002(1):5-9.
[27] Devine M D. Mechanisms of resistance to acetyl-coenzyme A carboxylase inhibitors: a review [J]. Pesticide Science, 1997,51(3):259-264.
[28] Christophye D. Weed resistance to Acetyl-coenzyme A carboxylase inhibitors: an update [J]. Weed Science, 2005, 53: 728-746.
[29] Cummins I, Wortley D J, Sabbadin F, et al. Key role for a glutathione transferase in multiple-herbicide resistance in grass weeds[J]. Proceedings of the National Academy of Sciences, 2013, 110(15): 5812-5817.
[30] Cummins I, Moss S, Cole D J, et al. Glutathione transferases in herbicide resistant and herbicide susceptible black-grass (Alopecurus myosuroides)[J]. Pesticide science, 1997, 51(3):244-250.