Abstract: In order to reduce the loss rate in rape picking up of segment harvesting, spring-finger cylinder pickup collector was installed in the indoor soil bin trolley, and the spring-finger cylinder type rape picking up test-bed was made through simulating field work environment. The regression equation's mathematical model of the loss rate in picking up which was influenced by the pick-up roller speed, the machine forward speed and the spring-finger into stubble depth was obtained through bench test and response surface analysis. The three factors listed in a descending order according to their importance were pick-up roller speed, machine forward speed and spring-finger into stubble depth. When the pick-up roller speed was 90 r/min, the machine forward speed was 0.7 m/s, and the spring-finger into stubble depth was 250 mm, the optimum picking up performance index was achieved.