Pathogen Identification and Biological Control of Gray Mold on Postharvest Tomato

doi:10.11924/j.issn.1000-6850.casb2020-0798

Abstract

Abstract:

In order to explore the biological control of gray mold caused by Botrytis cinerea on postharvest tomato, strain FQ-2 was isolated from tomato fruit by the Koch’s postulate, and the pathogen was identified based on its morphology and rDNA-ITS sequence. Bacillus amyloliquefaciens B9601-Y2 (Y2), an endophyte of wheat and B. velezensis DJB5 (DJB5), an endophyte of Dendrobium thyrsiflorum were used for biological control of the disease. By analyses, FQ-2 was identified as Botrytis cinerea, Y2 and DJB5 had good preventive and therapeutic effects on gray mold of tomato. Y2 with 1×10⁷cfu/mL and DJB5 with 1×10⁸cfu/mL had 88.10% and 73.47% of prevention effects, respectively, after 10 days when they were inoculated following FQ-2 in the same day. In the curing experiment, on the day of inoculation of B. cinerea, Y2 with 1×10⁸cfu/mL and DJB5 with 1×10⁷cfu/mL had 68.56% and 78.74% of curing effect, respectively, after 10 days when they were inoculated following FQ-2 in the same day. These results are of great significance to the postharvest biological control of tomato gray mold.

Key words: tomato, biological control, gray mold, Botrytis cinerea, Bacillus amyloliquefaciens, Bacillus velezensis, pathogen identification

CLC Number:

S432.1

Shen Yan, He Pengbo, He Pengfei, Wu Yixin, Kong Baohua, Li Xingyu, Shahzad Munir, He Yueqiu. Pathogen Identification and Biological Control of Gray Mold on Postharvest Tomato[J]. Chinese Agricultural Science Bulletin, 2021, 37(13): 102-107.

Figures/Tables 8

References 30

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浓度	生防菌株	CK	0 d	1 d	2 d	3 d	4 d	5 d
1×10⁶ cfu/mL	Y2	73.50±19.13aA	24.00±22.04cA	46.25±21.27bA	47.25±17.36bB	55.00±23.34bB	56.00±19.37bB	59.25±22.96bA
1×10⁶ cfu/mL	DJB5	73.50±19.13aA	27.50±21.18cA	53.75±17.76bA	56.00±20.17bA	56.50±25.40bAB	58.25±19.14bB	63.0±25.77abB
1×10⁷ cfu/mL	Y2	73.50±19.13aA	8.75±10.11eB	38.75±12.86dA	50.00±22.42cB	50.25±10.94cB	57.25±13.62bcB	61.00±23.88bA
1×10⁷ cfu/mL	DJB5	73.50±19.13aA	35.50±27.81dA	47.00±16.26cAB	57.00±11.29cA	69.50±14.77bA	73.25±12.28bA	85.00±12.14aA
1×10⁸ cfu/mL	Y2	73.50±19.13aA	23.00±24.68cA	47.25±14.28bA	63.25±12.69aA	67.50±12.30aA	69.00±14.01aA	69.75±12.29aA
1×10⁸ cfu/mL	DJB5	73.50±19.13aA	19.50±22.35dA	37.50±15.26cB	50.75±24.88bA	52.00±23.80bB	61.00±15.61abB	62.75±23.37abB

浓度	生防菌株	CK	0 d	1 d	2 d	3 d	4 d	5 d
1×10⁶ cfu/mL	Y2	73.50±19.13aA	24.00±22.04cA	46.25±21.27bA	47.25±17.36bB	55.00±23.34bB	56.00±19.37bB	59.25±22.96bA
1×10⁶ cfu/mL	DJB5	73.50±19.13aA	27.50±21.18cA	53.75±17.76bA	56.00±20.17bA	56.50±25.40bAB	58.25±19.14bB	63.0±25.77abB
1×10⁷ cfu/mL	Y2	73.50±19.13aA	8.75±10.11eB	38.75±12.86dA	50.00±22.42cB	50.25±10.94cB	57.25±13.62bcB	61.00±23.88bA
1×10⁷ cfu/mL	DJB5	73.50±19.13aA	35.50±27.81dA	47.00±16.26cAB	57.00±11.29cA	69.50±14.77bA	73.25±12.28bA	85.00±12.14aA
1×10⁸ cfu/mL	Y2	73.50±19.13aA	23.00±24.68cA	47.25±14.28bA	63.25±12.69aA	67.50±12.30aA	69.00±14.01aA	69.75±12.29aA
1×10⁸ cfu/mL	DJB5	73.50±19.13aA	19.50±22.35dA	37.50±15.26cB	50.75±24.88bA	52.00±23.80bB	61.00±15.61abB	62.75±23.37abB

浓度	生防菌株	CK	0 d	1 d	2 d	3 d	4 d	5 d
1×10⁶ cfu/mL	Y2	83.50±24.77Aa	31.75±16.49dAB	39.25±19.49cdAB	34.50±15.72cdB	45.50±18.20bcA	52.50±24.03bA	77.25±13.81aA
1×10⁶ cfu/mL	DJB5	83.50±24.77Aa	20.00±15.47dB	43.00±23.36cA	43.50±14.97cA	44.00±16.35cA	51.50±8.60bcA	63.00±20.55bA
1×10⁷ cfu/mL	Y2	83.50±24.77Aa	40.50±26.45bA	51.00±28.77bA	51.00±31.44bA	51.75±17.72bA	53.50±15.31bA	81.50±14.87aA
1×10⁷ cfu/mL	DJB5	83.50±24.77Aa	17.75±14.82dB	37.50±20.23cA	38.00±19.63cA	38.50±20.59cA	50.00±13.48cA	61.50±17.25bA
1×10⁸ cfu/mL	Y2	83.50±24.77Aa	26.25±27.95cB	28.75±18.91cB	30.50±17.01cB	45.00±17.32bA	56.50±16.94bA	75.55±21.28aA
1×10⁸ cfu/mL	DJB5	83.50±24.77Aa	28.00±19.04dA	32.50±29.18dA	35.50±15.04dA	41.00±25.11cdA	51.75±14.98bcA	69.25±14.35bA

浓度	生防菌株	CK	0 d	1 d	2 d	3 d	4 d	5 d
1×10⁶ cfu/mL	Y2	83.50±24.77Aa	31.75±16.49dAB	39.25±19.49cdAB	34.50±15.72cdB	45.50±18.20bcA	52.50±24.03bA	77.25±13.81aA
1×10⁶ cfu/mL	DJB5	83.50±24.77Aa	20.00±15.47dB	43.00±23.36cA	43.50±14.97cA	44.00±16.35cA	51.50±8.60bcA	63.00±20.55bA
1×10⁷ cfu/mL	Y2	83.50±24.77Aa	40.50±26.45bA	51.00±28.77bA	51.00±31.44bA	51.75±17.72bA	53.50±15.31bA	81.50±14.87aA
1×10⁷ cfu/mL	DJB5	83.50±24.77Aa	17.75±14.82dB	37.50±20.23cA	38.00±19.63cA	38.50±20.59cA	50.00±13.48cA	61.50±17.25bA
1×10⁸ cfu/mL	Y2	83.50±24.77Aa	26.25±27.95cB	28.75±18.91cB	30.50±17.01cB	45.00±17.32bA	56.50±16.94bA	75.55±21.28aA
1×10⁸ cfu/mL	DJB5	83.50±24.77Aa	28.00±19.04dA	32.50±29.18dA	35.50±15.04dA	41.00±25.11cdA	51.75±14.98bcA	69.25±14.35bA