Multidrug Resistance Detection of Strawberry Gray Mold Strains in Jiangsu Province

doi:10.11924/j.issn.1000-6850.casb2021-1013

Abstract

Abstract:

The purpose is to clarify the resistance level of Botrytis cinerea to common fungicides in main strawberry producing areas of Jiangsu Province. The resistance of 236 strains of Botrytis cinerea collected in 2019 and 2020 to 13 fungicides were measured by discriminative dose method, and the resistant frequency and multidrug resistance of the strains were analyzed. The results showed that all strains of Botrytis cinerea were sensitive to fludioxonil (Flu), but resistant to carbendazim (Car), pyraclostrobin (Pyra), iprodione (Ipr), procymidone (Pro), pyrimethanil (Pyr), and their resistant frequencies were 58.05%, 55.01%, 51.44%, 45.80%, and 33.94%, respectively. Strains of Botrytis cinerea had different resistance levels to different fungicides in different regions, the resistant strains were the dominant species in Lishui, Kunshan, Jiangyan and Tongshan, and the sensitive strains were the main species in Wuxi and Changzhou. According to the distribution of inhibitive rate of each fungicide, the tested fungicides were divided into three types: sensitive, sensitive decrease and resistant. A total of 9 resistant types were detected in this study, including Pyra^R(7.32%), Car^R(6.50%), Pyr^R(0.81%), Car^R Pyr^R (3.25%), Car^R Pyra^R(14.63%), Car^R Bos^R(1.63%), Car^R Pyra^RPyr^R (23.58%), Car^RBos^RPyra^R(7.31%), and Car^RBos^RPyra^RPyr^R (34.15%). The Botrytis cinerea strains of strawberry in Jiangsu Province basically have lost sensitivity to carbendazim and pyraclostrobin, and are sensitive decrease to iprodione, procymidone, pyramidamide and tebuconazole. Car^RPyra^RPyr^Rand Car^RBos^RPyra^R Pyr^R are the dominant populations. It is recommended to use different types of fungicides to control Botrytis cinerea alternately in the resistance management measures.

Key words: strawberry, Botrytis cinerea, fungicides, multidrug resistance, Jiangsu Province

CLC Number:

S482.2

XIAO Ting, QIAN Rongming, ZHANG Furong, ZHOU Bin, WU Huiqiu, JI Sen, SONG Junxian, ZHUANG Yiqing, YANG Jinghui. Multidrug Resistance Detection of Strawberry Gray Mold Strains in Jiangsu Province[J]. Chinese Agricultural Science Bulletin, 2022, 38(14): 110-117.

Figures/Tables 6

References 26

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序号	通用名	剂型	浓度	厂家	区分计量/(mg/L)
1	咪鲜胺	WP	50%	江苏省绿盾质保农药实验有限公司	5
2	多菌灵	WP	50%	江苏蓝丰生物化学有限公司	10
3	腐霉利	WP	50%	住友化学投资（中国）有限公司	10
4	戊唑醇	SC	430 g/L	拜耳（中国）有限公司	5
5	啶酰菌胺	WDG	50%	先正达（苏州）作物保护公司	10
6	氟吡菌酰胺	SC	50%	拜耳（中国）有限公司	5
7	氟唑菌酰胺	SC	62.5%	拜耳（中国）有限公司	5
8	环酰菌胺	原药	98.2%	沈阳化工研究院	5
9	扑海因	SC	50%	拜耳（中国）有限公司	10
10	吡唑醚菌酯	SC	25%	德国巴斯夫（中国）有限公司	10
11	嘧霉胺	WDG	80%	拜耳（中国）有限公司	5
12	嘧菌环胺	WDG	50%	先正达（苏州）作物保护公司	5
13	咯菌腈	WP	50%	先正达（苏州）作物保护公司	0.5

序号	通用名	剂型	浓度	厂家	区分计量/(mg/L)
1	咪鲜胺	WP	50%	江苏省绿盾质保农药实验有限公司	5
2	多菌灵	WP	50%	江苏蓝丰生物化学有限公司	10
3	腐霉利	WP	50%	住友化学投资（中国）有限公司	10
4	戊唑醇	SC	430 g/L	拜耳（中国）有限公司	5
5	啶酰菌胺	WDG	50%	先正达（苏州）作物保护公司	10
6	氟吡菌酰胺	SC	50%	拜耳（中国）有限公司	5
7	氟唑菌酰胺	SC	62.5%	拜耳（中国）有限公司	5
8	环酰菌胺	原药	98.2%	沈阳化工研究院	5
9	扑海因	SC	50%	拜耳（中国）有限公司	10
10	吡唑醚菌酯	SC	25%	德国巴斯夫（中国）有限公司	10
11	嘧霉胺	WDG	80%	拜耳（中国）有限公司	5
12	嘧菌环胺	WDG	50%	先正达（苏州）作物保护公司	5
13	咯菌腈	WP	50%	先正达（苏州）作物保护公司	0.5