Biological Control of Tobacco Root-knot Nematode Disease by Penicillium purpurogenum K1

doi:10.11924/j.issn.1000-6850.casb2022-0106

Abstract

Abstract:

In order to evaluate the biological control potential of Penicillium purpurogenum against tobacco root-knot nematode disease, the activity of P. purpurogenum K1 against the second stage juveniles and eggs of Meloidogyne incognita were tested in laboratory, and the control effect of P. purpurogenum K1 on tobacco root-knot nematode disease was tested in 2 fields for 2 years. The results were obtained as follows. (1) P. purpurogenum K1 strain had strong virulent activity against M. incognita. The corrected mortality rate of the second stage juveniles of M. incognita at 48 h was 94.53%, and the relative inhibition efficiency of egg hatching was 90.75%. (2) The control effects of P. purpurogenum with three application rates on tobacco root-knot nematode disease, and the comprehensive agronomic traits, yield and quality, and average price of flue-cured tobacco were better than those of Paecilomyces lilacinus treatment with the application rate of 33.00 kg/hm². The control effect of P. purpurogenum at the rate of 41.25 kg/hm² was the best with an average control effect of 80.60%, and the comprehensive agronomic traits, output value and average price of tobacco under this treatment were the best. The tobacco leaf yield, output value and average price were 2119.50 kg/hm², 56762.77 yuan/hm² and 26.78 yuan/kg, respectively. Therefore, P. purpurogenum K1 has great potential of biological control of tobacco root-knot nematode in field, and it can be developed and applied as biocontrol fungi against tobacco root-knot nematode disease in Panzhihua. The recommended application method is using 41.25 kg/hm² P. purpurogenum K1 at tobacco transplanting.

Key words: root-knot nematodes, tobacco, Penicillium purpurogenum, biological control, application rate

CLC Number:

S435.72

YAN Fangfang, KONG Chuixu, ZHANG Yingjie, MAO Min, JIAN Lianjun, WANG Rong. Biological Control of Tobacco Root-knot Nematode Disease by Penicillium purpurogenum K1[J]. Chinese Agricultural Science Bulletin, 2022, 38(33): 103-108.

Figures/Tables 7

References 28

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处理	24 h		48 h
处理	致死率/%	校正致死率/%	致死率/%	校正致死率/%
K1	85.00±0.82	84.79±0.89	94.67±1.25	94.53±1.25
对照	1.33±0.47	—	2.67±0.47	—

处理	24 h		48 h
处理	致死率/%	校正致死率/%	致死率/%	校正致死率/%
K1	85.00±0.82	84.79±0.89	94.67±1.25	94.53±1.25
对照	1.33±0.47	—	2.67±0.47	—

处理	孵化数量	相对抑制率/%
产紫青霉K1	16.67±1.25	90.75±0.87
无菌水对照	180.67±5.79	-

处理	孵化数量	相对抑制率/%
产紫青霉K1	16.67±1.25	90.75±0.87
无菌水对照	180.67±5.79	-

用量/(kg/hm²)	株高/cm	茎围/cm	最大叶长/cm	最大叶宽/cm	最大叶面积/cm²
3.00	93.6±1.1 bBC	9.6±0.3 bBC	62.8±1.3 bAB	32.1±0.8 bB	1283.8±29.4 bB
41.25	108.8±1.8 aA	10.4±0.2 aAB	64.7±2.7 abAB	36.2±1.7 aA	1491.3±72.2 aA
49.50	105.2±3.3 aB	10.5±0.2 aA	68.1±2.6 aA	31.9±0.5 bB	1356.6±46.4 bAB
0.00(CK1)	85.2±3.8 cC	8.4±0.2 cD	56.5±1.1 Cc	23.4±1.5 cC	823.7±22.5 cC
33.00(CK2)	90.2±2.0 bC	8.9±0.7 cCD	61.5±3.3 bBC	30.9±2.2 bB	1271.9±136.1 bB