Effects of Spraying Microelement-fertilizer on Physiological Metabolism of Tobacco Plant at Mature Stage Under Localized Fertilization

doi:10.11924/j.issn.1000-6850.casb2021-1024

Abstract

Abstract:

To explore the effect of foliar application of microelement-fertilizer on the maturity of tobacco leaves and improve tobacco leaf quality, ‘CB-1’ was selected as the experimental material. After 10 years’ localized fertilization with pure chemical fertilizer in tobacco fields, the effects of different medium and trace elements on the physiological metabolism of tobacco leaves were determined by foliar application 10 days and 20 days after topping of tobacco plants. The results showed that after spraying different medium and trace elements on leaves, the total content of chlorophyll a, chlorophyll b and total chlorophyll increased significantly, the leaf photosynthetic rate, transpiration rate and stomatal conductance increased significantly, the fluorescence parameters Fo and Fo/Fm decreased significantly, and Fm, Fv, Fv/Fm and Fv/Fo increased significantly. Nitrate reductase activity increased significantly. The relative permeability of cell membrane decreased significantly. Both CAT and SOD maintained high activities, while MDA content decreased significantly. The results indicated that spraying medium and trace elements in the late growth stage of flue-cured tobacco in the field with long-term pure chemical fertilizer application was beneficial to improving the stability of cell membrane structure, reducing the degree of membrane lipid peroxidation, maintaining the stability of photosynthetic structure, reducing the degradation of chloroplast pigments, improving the efficiency of light energy conversion, and slowing down the damage of reactive oxygen species. Spraying microelement-fertilizer is conducive to improving the photosynthetic capacity of tobacco leaves, alleviating the injury and aging of tobacco leaves, and improving the flue-curing adaptability of upper leaves.

Key words: flue-cured tobacco, medium and trace elements, localized fertilization, physiological metabolism, photosynthesis

CLC Number:

S572

WANG Yuemin, KE Yuqin, XIE Rongrong, LI Chunying, LI Wenqing. Effects of Spraying Microelement-fertilizer on Physiological Metabolism of Tobacco Plant at Mature Stage Under Localized Fertilization[J]. Chinese Agricultural Science Bulletin, 2022, 38(31): 24-30.

Figures/Tables 8

References 38

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微量元素处理	Chla	Chlb	Chl
CK	2.807bB	1.119bB	3.927bB
0.2%硫酸锌	3.347aA	1.306aA	4.653aA
0.1%钼酸	3.131abAB	1.239aAB	4.371abAB
0.1%硫酸锰	3.0801abAB	1.206abAB	4.286abAB
0.5%硫酸镁	3.0621abAB	1.236abAB	4.298abAB
0.5%硫酸亚铁	2.946bAB	1.203abAB	4.149bAB

微量元素处理	Chla	Chlb	Chl
CK	2.807bB	1.119bB	3.927bB
0.2%硫酸锌	3.347aA	1.306aA	4.653aA
0.1%钼酸	3.131abAB	1.239aAB	4.371abAB
0.1%硫酸锰	3.0801abAB	1.206abAB	4.286abAB
0.5%硫酸镁	3.0621abAB	1.236abAB	4.298abAB
0.5%硫酸亚铁	2.946bAB	1.203abAB	4.149bAB

微量元素处理	Pn/[µmolCO₂/(m²·s)]	Tr/[mmol H₂O/(m²·s)]	Gs/[mmolH₂O/(m²·s)]	Ci/(µmol/mol)
CK	2.58dC	0.931dC	72.364cD	382.980aA
0.2%硫酸锌	11.756aA	3.114bA	224.210aA	265.250cdBCD
0.1%钼酸	10.517bA	2.523cB	189.156bBC	244.375dD
0.1%硫酸锰	11.017abA	3.076bA	218.281aAB	259.250cdCD
0.3%硫酸镁	11.225abA	3.363aA	223.811aA	280.250bcBC
0.5%硫酸亚铁	8.267cB	2.581cB	180.072bC	292.750bB

微量元素处理	Pn/[µmolCO₂/(m²·s)]	Tr/[mmol H₂O/(m²·s)]	Gs/[mmolH₂O/(m²·s)]	Ci/(µmol/mol)
CK	2.58dC	0.931dC	72.364cD	382.980aA
0.2%硫酸锌	11.756aA	3.114bA	224.210aA	265.250cdBCD
0.1%钼酸	10.517bA	2.523cB	189.156bBC	244.375dD
0.1%硫酸锰	11.017abA	3.076bA	218.281aAB	259.250cdCD
0.3%硫酸镁	11.225abA	3.363aA	223.811aA	280.250bcBC
0.5%硫酸亚铁	8.267cB	2.581cB	180.072bC	292.750bB

微量元素处理	Fo	Fm	Fv	Fo/Fm	Fv/Fm	Fv/Fo
CK	11258.333aA	18880.333bB	8402.000dC	0.574aA	0.426cB	0.783cC
0.2%硫酸锌	8225.000bB	23927.333aA	16649.333aA	0.306bB	0.694aA	2.094aA
0.1%钼酸	8585.667bAB	22608.333aA	15318.667abAB	0.366bB	0.585bA	1.555bB
0.1%硫酸锰	8888.333bAB	22069.333aAB	14238.000abcABC	0.357bB	0.643abA	1.640bB
0.5%硫酸镁	8963.333bAB	22410.333aAB	12636.000bcABC	0.373bB	0.627abA	1.609bB
0.5%硫酸亚铁	9328.667abAB	22819.333aA	11293.333cdBC	0.347bB	0.616abA	2.023aA