Changes of Physical and Chemical Indexes of Tobacco Leaves Sealed with Hypoxia After Unsealing

doi:10.11924/j.issn.1000-6850.casb2021-0418

Abstract

Abstract:

After recuring, tobacco leaves usually have some qualities that cannot meet the taste requirement of the market, enterprises usually need to mellow the redried tobacco leaves for a period of time to coordinate the internal chemical components of tobacco leaves and improve the quality, and the technology of tobacco leaves sealed with hypoxia is often used. This study explored the changes of iodine value, pH, color difference value, polyphenol oxidase activity, appearance quality and sensory quality of tobacco leaves within 3 months after sealing, aiming to lay a foundation for the storage management, quality assurance and use of the tobacco leaves. The results showed that the iodine value of tobacco leaves gradually increased within 3 months after the unsealing, and the pH dropped rapidly within 1 month of unsealing and gradually remained stable after 2-3 months, the color difference values fluctuated, and the activity of polyphenol oxidase gradually increased. In addition, the polyphenol oxidase activity of the samples sealed for 3 months under hypoxia was significantly lower than those of the samples under natural storage for 3 months after unsealing, the pH value and polyphenol oxidase activity also had a smaller change range than that of the unsealing samples, and the change range was close to that under natural storage for 1 month after unsealing. Appearance color evaluation showed that natural storage for 3 months after unsealing had the greatest impact on the color value of Hunan Xiangxi B2F tobacco leaves, followed by the impact on aroma and chromaticity, but had no effect on the uniformity and oil content of the tobacco leaves. Besides, natural storage had a relatively great impact on the color, uniformity and chromaticity of Yunnan Wenshan C3F, and basically had no effect on aroma and oil content. And the two storage methods, natural storage for 3 months after unsealing and continuous sealed with hypoxia for 3 months, had almost no effect on the appearance color evaluation scores of tobacco leaves. The study evaluates the properties’ change of tobacco leaves stored in hypoxia after unsealing and lay a foundation for the processing and application of tobacco leaves stored in hypoxia after unsealing.

Key words: redried tobacco, sealed with hypoxia, iodine value, pH, polyphenol oxidase activity, color difference value

CLC Number:

TS41+1

HUNAG Lei, LI Yimeng, ZHONG Shuai, JIN Baofeng, GAO Jinjun, WANG Xiaoyuan, GUAN Luohao, WANG Chuliang, HUANG Wanting, LI Min. Changes of Physical and Chemical Indexes of Tobacco Leaves Sealed with Hypoxia After Unsealing[J]. Chinese Agricultural Science Bulletin, 2022, 38(4): 107-112.

Figures/Tables 7

References 26

[1]	管恩森, 孔芳芳, 王大海, 等. 烤烟自然醇化和人工醇化过程中感官质量的变化[J]. 中国烟草科学, 2020(2):54-58.
[2]	SUN J G, HE J W, WU F G, et al. Comparative analysis on chemical components and sensory quality of aging flue-cured tobacco from four main tobacco areas of China[J]. Agricultural sciences in China, 2011(8):1222-1231.
[3]	范坚强, 宋纪真, 赖成连, 等. 密封降氧抑制醇化过程中烟片颜色转深试验[J]. 烟草科技, 2005(12):23-26.
[4]	彭琛, 余其昌, 林锐峰, 等. 一种广东地区低氧贮存烟叶的夏秋季启用方法[P]. 中国专利,CN107495458A. 2017.
[5]	彭琛, 林锐峰, 任胜超, 等. 富氮控氧法对3种虫态烟草甲杀灭效果[J]. 中国农学通报, 2017, 33(31):122-126.
[6]	WU S, GUO Y, JOAN H I, et al. iTRAQ-based comparative proteomic analysis reveals high temperature accelerated leaf senescence of tobacco (Nicotiana tabacum L.) during flue-curing[J]. Genomics, 2020, 112(5).
[7]	MAHDAVI B, YAACOB W A, DIN L B. Phytochemical study of medicinal smokes from Etlingera brevilabrum leaves[J]. Journal of herbal medicine, 2018, 13:52-62. doi: 10.1016/j.hermed.2018.04.001 URL
[8]	WANG L, CHENG B, LI Z, et al. Intelligent tobacco flue-curing method based on leaf texture feature analysis[J]. Optik, 2017, 150:117-130. doi: 10.1016/j.ijleo.2017.09.088 URL
[9]	STANFILL S, CROUCHER R, GUPTA P, et al. Chemical characterization of smokeless tobacco products from South Asia: Nicotine, unprotonated nicotine, tobacco-specific N’-nitrosamines, and flavor compounds[J]. Food & chemical toxicology, 2018, 118:626-634.
[10]	李琳, 刘洋, 王豹祥, 等. 不同品种烟叶碘值吸光度值与评吸质量的相关性分析[J]. 江西农业学报, 2011, 23(10):94-96.
[11]	中国烟草标准化研究中心, 广东中烟工业公司, 郑州烟草研究院, 等. YC/T 222—2007,烟草及烟草制品 pH的测定[S]. 北京: 中国标准出版社, 2007:2-8.
[12]	陈飞程, 白金莹, 杨洋, 等. 不同成熟度烤烟色差参数与烘烤特性关系研究[J]. 江西农业学报, 2021, 33(2):78-82.
[13]	兰俊荣, 靖军领, 黄一兰, 等. 不同成熟度烟叶烘烤过程中多酚氧化酶活性变化[J]. 现代农业科技, 2010(23):54-55,57.
[14]	中国烟叶生产购销公司. GB 2635—1992,烤烟[S]. 北京:国家技术监督局, 1992:1-16.
[15]	司辉, 闫铁军, 张环宇, 等. 不同生态产区烤烟片烟化学成分在醇化中的变化规律研究[J]. 安徽农业科学, 2011, 39(22):13328-13333.
[16]	RAJAN A G, SIVASUBRAMANIAN M, GOWTHAMAN S, et al. Investigation of physical and chemical properties of tobacco seed oil fatty acid methyl ester for biodiesel production[J]. Materials today: proceedings, 2021(1).
[17]	谭小红. 复烤片烟自然醇化过程中品质变化研究[J]. 南方农机, 2016(47):77.
[18]	刘春奎, 贾琳, 王晓宾, 等. 广东烤烟总挥发碱、总挥发酸和pH的分析[J]. 南方农业学报, 2014, 45(9):1647-1651.
[19]	胡亚杰, 韦建玉, 宗钊辉, 等. 云南不同烟区片烟醇化过程色度及主要化学成分变化特征研究[J]. 中国农学通报, 2020, 36(22):143-153.
[20]	宗钊辉, 刘高, 韦建玉, 等. 基于色差值和色度值的醇化片烟褐变判定模型研究[J]. 南方农业学报, 2018, 49(10):2040-2046.
[21]	FERNANDES G M, SILVA W R, BARRETO D N, et al. Novel approaches for colorimetric measurements in analytical chemistry- A review[J]. Analytica chimica acta, 2020, 1135:187-203.
[22]	杨树勋. 烟草酶促棕色化反应机理及其调控研究进展[J]. 作物研究, 2019, 33(3):246-250.
[23]	KEPPLER J K, SCHWARZ K, GOOT A. Covalent modification of food proteins by plant-based ingredients (polyphenols and organosulphur compounds): A commonplace reaction with novel utilization potential[J]. Trends in food science & technology, 2020, 101:38-49.
[24]	宋怡, 夏建军, 丁中涛. 抗坏血酸对烟草中多酚类物质稳定性影响的研究[J]. 云南化工, 2008, 35(6):12-14.
[25]	CHEN J, LI Y, HE X, et al. Influences of different curing methods on chemical compositions in different types of tobaccos[J]. Industrial crops and products, 2021, 167(1):113534. doi: 10.1016/j.indcrop.2021.113534 URL
[26]	任胜超, 彭琛, 林锐峰, 等. 物理气调法片烟养护及醇化效果研究[J]. 中国农学通报, 2018, 34(8):95-103.

样品组	B2F		C3F
样品组	455 nm	483 nm	455 nm	483 nm
解封当时	0	0	0	0
解封1个月	-2.04	-5.56	2.38	1.32
解封2个月	7.69	7.29	3.33	0.92
解封3个月	9.35	8.74	9.13	11.61
低氧密封3个月	4.38	-0.66	3.23	6.07

样品组	B2F		C3F
样品组	455 nm	483 nm	455 nm	483 nm
解封当时	0	0	0	0
解封1个月	-2.04	-5.56	2.38	1.32
解封2个月	7.69	7.29	3.33	0.92
解封3个月	9.35	8.74	9.13	11.61
低氧密封3个月	4.38	-0.66	3.23	6.07

样品组	B2F	C3F
解封当时	0	0
解封1个月	1.65	1.57
解封2个月	1.99	2.05
解封3个月	1.78	2.05
低氧密封3个月	0.34	1.02

样品组	B2F	C3F
解封当时	0	0
解封1个月	1.65	1.57
解封2个月	1.99	2.05
解封3个月	1.78	2.05
低氧密封3个月	0.34	1.02

物料名称	解封时间	外观质量评价指标						综合得分
物料名称	解封时间	颜色	闻香	均匀度	身份	油分	色度	综合得分
2017/湖南湘西/B2F	当天	7.000	7.000	7.000	8.000	7.000	7.000	7.100
	1月后	6.000	6.500	7.000	8.000	7.000	6.500	6.675
	2月后	6.000	6.500	7.000	8.000	7.000	6.500	6.675
	3月后	5.500	6.500	7.000	8.000	7.000	6.500	6.550
	低氧密封3个月	5.750	6.500	7.000	8.000	7.000	6.500	6.613
2017/云南文山/C3F	当天	7.000	7.000	7.000	8.000	7.000	7.000	7.100
	1月后	6.500	7.000	6.500	8.000	7.000	6.500	6.825
	2月后	6.500	7.000	6.500	8.000	7.000	6.500	6.825
	3月后	6.500	7.000	6.500	8.000	7.000	6.500	6.825
	低氧密封3个月	6.500	7.000	6.500	8.000	7.000	6.500	6.825