Intestine of Acanthopagrus schlegelii and Microbial Communities in the Aquatic Water: Study in Two Culture Modes

doi:10.11924/j.issn.1000-6850.casb2020-0324

Abstract

Abstract:

To investigate the microbial communities’ structure of water and intestine of black sea bream (Acanthopagrus schlegelii) reared in in-pond raceway culture system (IP) and pond (EP), intestines and culture water were selected as research objects. Using high-throughput sequencing technology, the structure and function of bacterial communities under the two different culture modes were compared and analyzed. The results show that the microbial communities are mainly composed of Proteobacteria, Actinobacteria, Cyanobacteria, Firmicutes, Bacteroidetes and Chloroflexi, and the sum of their abundance is 98.32%-99.22%. At genus level, bacteria are significantly different. Furthermore, the main increased species in IP mode were Ruegeria (14.84%) Synechococcus (14.24%), Marivita (3.58%), norank_o__PeM15 (2.97%) and norank_C__KD4-96 (2.56%)(P<0.05) (IP > EP). However, the main bacteria in EP model are Enterobacter (10.31%), Mycobacterium (10.30%), Romboutsia (7.37%), Enterococcus (6.29%) and Lactococcus (5.38%) (P<0.05) (IP < EP). The bacterial community in intestine has high richness and diversity, IP mode is significantly higher than that in EP mode (P<0.05), but there is no significant difference between fish intestines (P>0.05). There are differences in the structure and function of the flora under the two culture modes, and there are a large proportion of bacterial communities with significant differences in relative abundance. Most of the dominant bacteria with significant differences are beneficial bacteria in the intestine of black sea bream in IP mode, while in the EP mode, the bacteria are mostly chronic or conditional pathogens.

Key words: in-pond raceway culture system, black sea bream, microbial community, high-throughput sequencing, intestine

CLC Number:

S964.9

Nie Zhijuan, Shao Nailin, Zhang Zhiwei, Hu Jiawen, Xu Pao, Xu Gangchun. Intestine of Acanthopagrus schlegelii and Microbial Communities in the Aquatic Water: Study in Two Culture Modes[J]. Chinese Agricultural Science Bulletin, 2021, 37(27): 155-164.

Figures/Tables 4

References 52

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