Single-cell Transcriptome Sequencing Technology and Its Application in Plant Research

doi:10.11924/j.issn.1000-6850.casb2021-0316

Abstract

Abstract:

In order to explore single-cell transcriptome sequencing technology (scRNA-seq) application in plant research, this review summarized the development status of scRNA-seq, and introduced its applications in embryonic development, immune cells, tumors and plant research. Secondly, this article analyzed the key technologies of scRNA-seq (ie. single-cell separation, single-cell whole transcriptome cDNA amplification technology and high-throughput sequencing). Finally, this article summed up the scRNA-seq applicable to plants based on the characteristics of plant cells, aiming to provide a theoretical basis for the better use of the method in plant research.

Key words: plant, single-cell transcriptome sequencing, transcriptomics, single-cell separation technology, high-throughput sequencing

CLC Number:

CHEN Liuhong, ZHAO Chunlei, WANG Xi, LI Yanli, DING Guangzhou, CHEN Li. Single-cell Transcriptome Sequencing Technology and Its Application in Plant Research[J]. Chinese Agricultural Science Bulletin, 2022, 38(3): 87-93.

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