Effects of Nano-silver Prepared from Tea Extract on Soil Microorganisms

doi:10.11924/j.issn.1000-6850.casb2022-0240

Abstract

Abstract:

To explore the effect of nano-silver prepared from tea extract on the biosafety of soil microorganisms, the nano-silver used as antibacterial materials were synthesized by plant reduction method, and prepared into 1000 mg/L nano-silver spray. Then, we analyzed nano-silver’s impact on soil microbial diversity and richness. The results showed that the population of microorganisms was similar in the soil samples of blank control group (CK) and experimental group (Nano-Ag), but the richness of microorganisms was different. In both the experimental group and the blank control group, the Coverage value was high (Coverage>99.71%), the Shannon index was high (Shannon>5.46), and the Simpson index was low (Simpson<0.02), while ACE index and Chao index had little difference between CK and Nano-Ag group (ACE<2, Chao<6). In addition, the nano-silver inhibited the growth of Proteobacteria (Alphaproteobacteria) and Actinobacteria (Thermoleophilia), but promoted the growth of Chloroflexi (Ktedonobacteria). This study verifies that nano-silver has a certain degree of biosafety, which will broaden the potential application scope of nano-sliver and lay a foundation for the further application of nanomaterials in environmental and medical fields.

Key words: nano-silver, biosafety, soil microorganisms, diversity

CLC Number:

HONG Ciqing, SUN Yuyao, MO Wenjing, FANG Yun, CHEN Fangrong, GUI Fangze, GUAN Xiong, PAN Xiaohong. Effects of Nano-silver Prepared from Tea Extract on Soil Microorganisms[J]. Chinese Agricultural Science Bulletin, 2022, 38(23): 56-63.

Figures/Tables 10

References 25

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扩增子	引物	引物序列	扩增区域	退火温度/℃
细菌16S	338F	ACTCCTACGGGAGGCAGCAG	338F_806R	55
细菌16S	806R	GGACTACHVGGGTWTCTAAT	338F_806R	55

扩增子	引物	引物序列	扩增区域	退火温度/℃
细菌16S	338F	ACTCCTACGGGAGGCAGCAG	338F_806R	55
细菌16S	806R	GGACTACHVGGGTWTCTAAT	338F_806R	55

成分	用量
5×缓冲液	4 μL
2.5 mM dNTPs	2μL
5 μM 正向引物	0.8μL
5 μM 反向引物	0.8μL
FastPfu 聚合酶	0.4μL
牛血清白蛋白	0.2μL
DNA 模板	10 ng
补ddH₂O至	20 μL

成分	用量
5×缓冲液	4 μL
2.5 mM dNTPs	2μL
5 μM 正向引物	0.8μL
5 μM 反向引物	0.8μL
FastPfu 聚合酶	0.4μL
牛血清白蛋白	0.2μL
DNA 模板	10 ng
补ddH₂O至	20 μL

循环步骤	温度/℃	时间	循环数
预变性	95	3 min	1
变形	95	30 s	27
退火	55	30 s
延伸	72	45 s
最终延伸	72	10 min	1
保存	10	+∞