Effect of Compound Biocontrol Agents on the Occurrence of Banana Fusarium Wilt

doi:10.11924/j.issn.1000-6850.casb19040058

Abstract

Abstract:

Fusarium wilt of banana, popularly known as Panama disease, is a lethal fungal disease caused by the soil-borne fungus Fusarium oxysporum f. sp. cubense (Foc). Recently, China’s banana production has been seriously threatened by the emergence of Fusarium wilt in the major production regions. It is believed that the biological control of this disease is an alternative to the chemical fungicides and other conventional control methods. The application potential of this biological control technique is greatly supported by our previous study, and the results of in vitro antagonistic activity test indicated that the combination of biocontrol agents (Trichoderma viride. XP1, Bacillus amyloliquefaciens. XP2) exhibited significant bacteriostatic effect on Foc. The objective of our current study is to further assay the effects of multiple biocontrol bacteria on Fusarium wilt through pot experiment, thereby providing the theoretical support for the biocontrol mechanism of Fusarium wilt. The experiment is a randomized complete block design with four treatments: control (CK) treatment: inoculating inactivated Foc4-GFP (100 mL)+ inactivated compound biological agent (500 mL), T1 treatment: inoculating Foc4-GFP (100 mL)+ inactivated compound biocontrol agent (500 mL), T2 treatment: inoculating Foc4-GFP (100 mL)+ compound biocontrol agent (100 mL)+ inactivated compound biocontrol agent (400 mL), T3 treatment: inoculating Foc4-GFP (100 mL)+ compound biocontrol agent (500 mL). The activities of antioxidant enzyme and cell wall degrading enzyme, content of nosotoxin, leaf number, plant height, cauloid diameter, root system length and disease index of banana seedlings were measured. The seedlings inoculated with mixed biocontrol bacteria had significantly lower activities of antioxidant enzyme, cell wall degrading enzyme, and the content of nosotoxin than those of control treatment (P<0.05). Meanwhile, the content of fusarium acid (FA), beauvericin (BEA) and enniatine (EB) of banana seedlings of T2 and T3 treatments were reduced by 93.31%, 93.64%, 94.71% and 94.56%, 95.76%, 97.87% compared with T1 treatment, respectively. The inoculation of mixed biocontrol bacteria showed positive effects on Fusarium wilt resistance and growth of banana plants, and the control efficiency of T2 and T3 treatments on banana Fusarium wilt was up to 81.7% and 95.0%, respectively. The biocontrol technique via inoculating mixed bacteria strains could be a good choice to control banana Fusarium wilt, reduce the activities of CAT, POD, PAL, CMC and PME, and remarkably decrease the content of nosotoxin in banana seedlings, achieving the control efficiency of 81.7%.

Key words: banana Fusarium wilt, biocontrol agents, enzyme activity, pathotoxin, control effect

CLC Number:

Sun Jie, Ma Fengjuan, Xie Kaizhi, Xu Peizhi, Gu Wenjie, Lu Yusheng, Li Xia, Sun Lili. Effect of Compound Biocontrol Agents on the Occurrence of Banana Fusarium Wilt[J]. Chinese Agricultural Science Bulletin, 2020, 36(16): 135-142.

Figures/Tables 6

References 42

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处理	CAT				POD				PAL
处理	1 d	3 d	5 d	7 d	1 d	3 d	5 d	7 d	1 d	3 d	5 d	7 d
CK	114.3c	114.6c	123.5c	115.9b	35.7c	40.6b	38.9c	32.2c	65.6c	46.8c	75.1c	64.3c
T1	153.6a	182.6a	189.9a	170.8a	48.1a	54.2a	54.9a	49.7a	96.9a	72.0a	122.8a	90.3a
T2	146.5a	162.6b	158.2b	158.2a	39.9b	43.0b	47.8b	35.6b	74.2b	53.2b	106.4b	74.5b
T3	125.4b	157.0b	139.9c	135.4b	36.7c	37.7c	45.7b	34.2b	66.7c	54.0b	81.4c	72.0b

处理	CAT				POD				PAL
处理	1 d	3 d	5 d	7 d	1 d	3 d	5 d	7 d	1 d	3 d	5 d	7 d
CK	114.3c	114.6c	123.5c	115.9b	35.7c	40.6b	38.9c	32.2c	65.6c	46.8c	75.1c	64.3c
T1	153.6a	182.6a	189.9a	170.8a	48.1a	54.2a	54.9a	49.7a	96.9a	72.0a	122.8a	90.3a
T2	146.5a	162.6b	158.2b	158.2a	39.9b	43.0b	47.8b	35.6b	74.2b	53.2b	106.4b	74.5b
T3	125.4b	157.0b	139.9c	135.4b	36.7c	37.7c	45.7b	34.2b	66.7c	54.0b	81.4c	72.0b

处理	CMC/[U/(mg·min)]				PME/[U/(H⁺ μmoles·h)]
处理	1 d	3 d	5 d	7 d	1 d	3 d	5 d	7 d
CK	1.51c	2.73c	2.94c	3.07b	43.1c	39.4b	40.4c	65.0c
T1	5.17a	8.79a	12.61a	11.96a	113.6a	157.6a	188.6a	200.0a
T2	3.74b	5.45b	8.43b	4.83a	63.7b	93.7b	130.1b	117.6b
T3	2.92c	3.31c	4.48c	3.05b	64.4b	72.5c	77.4b	111.0b

处理	CMC/[U/(mg·min)]				PME/[U/(H⁺ μmoles·h)]
处理	1 d	3 d	5 d	7 d	1 d	3 d	5 d	7 d
CK	1.51c	2.73c	2.94c	3.07b	43.1c	39.4b	40.4c	65.0c
T1	5.17a	8.79a	12.61a	11.96a	113.6a	157.6a	188.6a	200.0a
T2	3.74b	5.45b	8.43b	4.83a	63.7b	93.7b	130.1b	117.6b
T3	2.92c	3.31c	4.48c	3.05b	64.4b	72.5c	77.4b	111.0b

处理	发病率/%	病情指数	防治效果/%
CK	0d	0d	—
T1	100a	65.3a	—
T2	18.3b	10.8b	81.7b
T3	5.0c	5.0c	95.0a