Effects of Melatonin Priming on Physiological Characteristics of Cucumber Seedlings Under Drought Stress

doi:10.11924/j.issn.1000-6850.casb2021-0781

Abstract

Abstract:

The purpose is to study the effect of melatonin (MT) seed priming on cucumber seedling growth under drought stress, so as to provide a theoretical basis for selecting MT as cucumber seed priming agent or coating agent. The seeds were treated with 50, 100, 150 μmol/L melatonin (MT) priming for 6, 12 and 15 h, and the unprimed seeds were used as a control, the drought stress was set at 50% of field moisture capacity, and the substrate pot cultivation was used. The results showed that 50-100 μmol/L of MT priming for 15 h triggered a significant increase in drought tolerance of cucumber seedlings. Compared with the control, 50 μmol/L MT priming increased the photosynthetic rate of the seedlings by 85.5%, and SOD, POD and CAT activities by 5.1%, 42.4% and 38.8%, respectively, and the root vigor was improved by 154.0%, indicating that suitable concentration of MT priming could improve the drought tolerance of cucumber seedlings by enhancing the antioxidant enzyme activities and root vigor of the seedlings.

Key words: cucumber, seed priming, melatonin, drought stress, autioxidant enzyme activity

CLC Number:

S642.2

YIN Shanshan, ZHOU Guoyan, GU Bowen, WU Chuncheng, YAN Liying, XIE Yang. Effects of Melatonin Priming on Physiological Characteristics of Cucumber Seedlings Under Drought Stress[J]. Chinese Agricultural Science Bulletin, 2022, 38(19): 30-36.

Figures/Tables 7

References 32

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处理	光合速率/[μmol/(m²·s)]	气孔导度/[mmol/(m²·s)]	蒸腾速率/[mmol/(m²·s)]	胞间CO₂浓度/(μmol/mol)
CK	6.34±0.75a	199.35±1.34cd	6.53±0.14a	408.67±16.33cd
A₀B₀	2.56±0.61c	153.30±7.39e	2.56±0.17d	481.10±8.20a
A₁B₁	3.11±0.54bc	158.60±14.57e	3.33±0.21c	437.23±13.18bcd
A₁B₂	3.03±0.49bc	320.10±37.19a	3.49±0.17c	417.63±20.20bcd
A₁B₃	4.82±1.20ab	286.75±0.92a	4.36±0.54b	401.77±20.71d
A₂B₁	3.51±1.26bc	182.30±8.20de	3.24±0.13c	445.57±7.28abc
A₂B₂	4.13±0.19bc	225.15±13.51bc	3.76±0.30bc	440.45±1.06bc
A₂B₃	4.75±1.65ab	243.20±21.39b	3.56±0.29c	420.53±16.00bcd
A₃B₁	2.67±0.55c	176.80±17.39de	2.56±0.56d	449.80±8.75ab
A₃B₂	2.72±0.08c	152.45±8.13e	1.82±0.06e	448.93±5.82ab
A₃B₃	3.00±0.04bc	160.70±13.01e	1.75±0.19e	442.13±43.27bc

处理	光合速率/[μmol/(m²·s)]	气孔导度/[mmol/(m²·s)]	蒸腾速率/[mmol/(m²·s)]	胞间CO₂浓度/(μmol/mol)
CK	6.34±0.75a	199.35±1.34cd	6.53±0.14a	408.67±16.33cd
A₀B₀	2.56±0.61c	153.30±7.39e	2.56±0.17d	481.10±8.20a
A₁B₁	3.11±0.54bc	158.60±14.57e	3.33±0.21c	437.23±13.18bcd
A₁B₂	3.03±0.49bc	320.10±37.19a	3.49±0.17c	417.63±20.20bcd
A₁B₃	4.82±1.20ab	286.75±0.92a	4.36±0.54b	401.77±20.71d
A₂B₁	3.51±1.26bc	182.30±8.20de	3.24±0.13c	445.57±7.28abc
A₂B₂	4.13±0.19bc	225.15±13.51bc	3.76±0.30bc	440.45±1.06bc
A₂B₃	4.75±1.65ab	243.20±21.39b	3.56±0.29c	420.53±16.00bcd
A₃B₁	2.67±0.55c	176.80±17.39de	2.56±0.56d	449.80±8.75ab
A₃B₂	2.72±0.08c	152.45±8.13e	1.82±0.06e	448.93±5.82ab
A₃B₃	3.00±0.04bc	160.70±13.01e	1.75±0.19e	442.13±43.27bc

指标	叶面积	SOD活性	POD活性	CAT活性	MDA含量	蒸腾速率	光合速率	气孔导度	胞间CO₂浓度	根系活力	叶绿素含量
叶面积	1
SOD活性	0.097	1
POD活性	-0.529	0.170	1
CAT活性	-0.089	0.213	0.330	1
MDA含量	-0.556	-0.011	-0.014	-0.302	1
蒸腾速率	0.205	0.499	-0.118	-0.476	-0.185	1
光合速率	0.419	0.506	-0.183	-0.296	-0.377	0.901^**	1
气孔导度	0.513	0.463	-0.173	0.083	-0.438	0.416	0.368	1
胞间CO₂浓度	-0.619^*	-0.568	0.003	0.154	0.555	-0.682^*	-0.740^**	-0.717^*	1
根系活力	0.466	0.559	-0.058	0.592	-0.520	0.151	0.346	0.539	-0.574	1
叶绿素	0.275	0.101	0.064	0.487	-0.445	-0.202	-0.050	0.291	-0.310	0.621^*	1

指标	叶面积	SOD活性	POD活性	CAT活性	MDA含量	蒸腾速率	光合速率	气孔导度	胞间CO₂浓度	根系活力	叶绿素含量
叶面积	1
SOD活性	0.097	1
POD活性	-0.529	0.170	1
CAT活性	-0.089	0.213	0.330	1
MDA含量	-0.556	-0.011	-0.014	-0.302	1
蒸腾速率	0.205	0.499	-0.118	-0.476	-0.185	1
光合速率	0.419	0.506	-0.183	-0.296	-0.377	0.901^**	1
气孔导度	0.513	0.463	-0.173	0.083	-0.438	0.416	0.368	1
胞间CO₂浓度	-0.619^*	-0.568	0.003	0.154	0.555	-0.682^*	-0.740^**	-0.717^*	1
根系活力	0.466	0.559	-0.058	0.592	-0.520	0.151	0.346	0.539	-0.574	1
叶绿素	0.275	0.101	0.064	0.487	-0.445	-0.202	-0.050	0.291	-0.310	0.621^*	1

处理	SOD活性	POD活性	CAT活性	MDA含量	叶绿素含量	根系活力	光合速率	气孔导度	蒸腾速率	胞间CO₂浓度	平均值	排序
CK	0.92	0.23	0.03	0.88	0.19	0.48	1.00	0.28	1.00	0.91	0.59	3
A₀B₀	0.62	0.11	0.53	0.00	0.00	0.07	0.00	0.01	0.17	0.00	0.15	11
A₁B₁	0.48	0.55	0.04	0.24	0.64	0.15	0.15	0.04	0.27	0.56	0.31	9
A₁B₂	0.70	0.15	0.47	0.89	0.63	0.66	0.12	1.00	0.26	0.80	0.57	4
A₁B₃	1.00	0.27	0.60	0.82	0.80	0.88	0.60	0.80	0.34	1.00	0.71	1
A₂B₁	0.00	0.00	0.00	0.86	0.13	0.00	0.25	0.18	0.17	0.47	0.21	10
A₂B₂	0.39	0.05	0.53	0.97	1.00	0.42	0.41	0.43	0.21	0.54	0.50	6
A₂B₃	0.70	0.33	0.89	1.00	0.64	1.00	0.58	0.54	0.17	0.78	0.66	2
A₃B₁	0.55	1.00	1.00	0.94	0.52	0.31	0.03	0.15	0.07	0.44	0.50	5
A₃B₂	0.50	0.14	0.98	0.87	0.98	0.97	0.04	0.00	0.01	0.46	0.50	7
A₃B₃	0.51	0.27	0.45	0.81	0.58	0.21	0.12	0.05	0.00	0.54	0.35	8