Effect of Tetranychus truncatus Feeding on Enzyme Activities of Solanum tuberosum

doi:10.11924/j.issn.1000-6850.casb2022-0060

Abstract

Abstract:

In order to clarify the effect of feeding stimulation of Tetranychus truncatus on enzyme activities of different drought-tolerant potato (Solanum tuberosum) varieties, two potato varieties (‘Netherlands No.15’, early maturing, drought-sensitive; ‘Longshu 10’, late-maturing, drought-tolerant) were inoculated with mites, and the activities of 5 defense enzymes (CAT, POD, PPO, PAL and LOX) in potato leaves were determined within 7 days. The results showed that the activities of CAT, POD, PPO, PAL and LOX increased to different degrees after the stimulation of mites, and showed a trend of first increase and then decrease in time sequence, and were significantly higher than those of CK (healthy plant without mites) after 3 days. All the 5 enzymes were involved in the defense response of plants to mites, so as to promote the reduction of free oxygen in the injured plants, and increase the contents of phenylpropanoids, such as lignin, anthocyanin and alkaloids, and hydroperoxides to enhance the defense ability of plants. 7 days after mite damage, the activities of the 5 enzymes significantly decreased, and were significantly lower than those of CK, indicating that leaf mites might lead to plant disorder after long-term damage, so the plants could not well maintain efficient defense responses to mite damage. The activities of CAT, PPO and PAL of the two potato varieties were analyzed at the same time after leaf mite damage, and the drought-sensitive potato variety showed high resistance to mites. Although the POD activity of drought-tolerant potato variety was higher after being fed by spider mites, the POD activity of drought-sensitive potato variety increased more. There was no significant difference of LOX activity between the two varieties. Overall analysis showed that drought-sensitive potato had higher resistance to mites than drought-tolerant varieties. The results can provide a theoretical basis for the scientific control of leaf mites and variety breeding of potato.

Key words: Solanum tuberosum, Tetranychus truncatus, defense enzymes, enzyme activity, defense response, mite resistance

CLC Number:

S435.32

MA Yue, ZHANG Kexin, WANG Fawu, ZHANG Qiangyan, ZHANG Yanlei, TAO Yan, LIU Huiping, LIU Changzhong. Effect of Tetranychus truncatus Feeding on Enzyme Activities of Solanum tuberosum[J]. Chinese Agricultural Science Bulletin, 2022, 38(16): 125-131.

Figures/Tables 5

References 24

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