Exogenous Selenium: Effects on Antioxidant Enzyme Activity, Yield and Selenium Content in Rice

doi:10.11924/j.issn.1000-6850.casb2020-0111

Abstract

Abstract:

The aim is to study the effects of two exogenous selenium applications on antioxidant enzyme activity, yield and selenium content of rice Oryza sativa L. cv. ‘Fuyuan No. 4’, determine the best way of selenium application and provide reference for developing selenium-rich agricultural products. In the low-selenium area of Ningxia (Yongning County), ‘Fuyuan No. 4’ was used as the experimental material, and selenium-rich micro-fertilizer capsule was used as soil base application, chelated selenium foliar fertilizer combined with selenium leaf fertilizer was sprayed at the key growth stage of rice. By means of mathematical statistics, the changes of antioxidant enzyme activity, yield and selenium content of rice treated with different exogenous selenium were analyzed. Respectively, MDA and POD activity of rice leaves were 41.17% and 35.98% higher than those of control at different growth stages, and significant differences were observed between the chelated selenium leaf fertilizer treatment and other treatments. The SOD activity in leaves increased first and then decreased, the difference between each treatment and the control group was significant, and that of the T3 treatment increased by 91.71%. The selenium content of stem, leaf and rice increased under different selenium treatments, the organic selenium content of rice accounted for 62.11%-80.96% of the total selenium content of rice. After variance analysis, the difference was significant, and the spraying effect was better than base application effect. From the heading stage to the filling stage, different exogenous selenium treatments had an effect on the activity of antioxidant enzymes in rice, which was related to the selenium concentration. In the low-selenium areas, exogenous selenium supplement could enrich selenium in rice plants, and the effect of applying selenium fertilizer on leaves was better than that of selenium enriched micro-fertilizer capsules. Moreover, the exogenous selenium treatment in the Se-deficient areas could improve the yield of rice to a certain extent, and increase the selenium content in rice leaves and grains.

Key words: exogenous selenium, rice, antioxidant enzyme activity, yield, selenium content

CLC Number:

S511.3+1

Ma Jie, Wang Hanlin, Hou Xiaoning, Jin rui, Yang Juan, Dang Wenrui, Wang Mingguo, Zheng Guoqi. Exogenous Selenium: Effects on Antioxidant Enzyme Activity, Yield and Selenium Content in Rice[J]. Chinese Agricultural Science Bulletin, 2021, 37(6): 9-15.

Figures/Tables 11

References 23

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处理	施肥方式	硒肥品种	施肥量
CK	---	不施硒肥	---
T1	基施	硒肥微肥胶囊	30 kg/hm²
T2	基施	硒肥微肥胶囊	60 kg/hm²
T3	孕穗期和抽穗期叶面喷施	螯合硒叶面肥	0.025 kg/hm²
T4	孕穗、抽穗和灌浆期叶面喷施	螯合硒叶面肥	0.02 kg/hm²

处理	施肥方式	硒肥品种	施肥量
CK	---	不施硒肥	---
T1	基施	硒肥微肥胶囊	30 kg/hm²
T2	基施	硒肥微肥胶囊	60 kg/hm²
T3	孕穗期和抽穗期叶面喷施	螯合硒叶面肥	0.025 kg/hm²
T4	孕穗、抽穗和灌浆期叶面喷施	螯合硒叶面肥	0.02 kg/hm²

	全硒/(μg/100g)	pH	有机质/(g/kg)	碱解氮/(mg/kg)	有效磷/(mg/kg)	速效钾/(mg/kg)
CK	21.98	7.95	17.20	65.72	17.57	166.17
T1	21.05	8.19	17.28	70.52	17.61	172.59
T2	21.83	8.39	18.35	69.08	19.70	159.86
T3	22.05	8.12	17.63	74.24	17.97	163.10
T4	22.84	8.21	16.10	76.52	22.61	161.94
F值	0.160	3.46	0.633	0.389	2.90	0.972

	全硒/(μg/100g)	pH	有机质/(g/kg)	碱解氮/(mg/kg)	有效磷/(mg/kg)	速效钾/(mg/kg)
CK	21.98	7.95	17.20	65.72	17.57	166.17
T1	21.05	8.19	17.28	70.52	17.61	172.59
T2	21.83	8.39	18.35	69.08	19.70	159.86
T3	22.05	8.12	17.63	74.24	17.97	163.10
T4	22.84	8.21	16.10	76.52	22.61	161.94
F值	0.160	3.46	0.633	0.389	2.90	0.972

	砂粒(0.05~2 mm) 所占比例/%	粉粒(0.01~0.05 mm) 所占比例/%	粘粒(0.001~0.01 mm) 所占比例/%	胶粒(<0.001 mm) 所占比例/%
CK	22.86	29.09	27.40	20.65
T1	14.13	34.81	29.07	21.99
T2	13.98	33.26	31.91	20.84
T3	15.41	30.72	29.84	24.03
T4	12.66	33.85	30.81	22.68
F值	1.705	2.098	1.194	0.143