Effects of Clcium Cyanamide and Its Combined Application with Biochar and Cake Fertilizer on Enzyme Activity and Total Microorganism Biomass in Tobacco Planting Soil

doi:10.11924/j.issn.1000-6850.casb2021-0030

Abstract

Abstract:

To investigate the effects of calcium cyanamide and its combination with biochar and cake fertilizer on soil enzyme activity and total microbial biomass in tobacco planting soil, four treatments were conducted, including 150 kg/hm² calcium cyanamide (T1), 150 kg/hm² calcium cyanamide combined with 600 kg/hm² biochar (T2), 150 kg/hm² calcium cyanamide combined with 600 kg/hm² cake fertilizer (T3) and conventional fertilization (CK, no application of calcium cyanamide), in Yong’an of Liuyang and Jiangyong of Yongzhou in Hunan Province. The results showed that in terms of enzyme activity, except the acid phosphatase activity of CK treatment was significantly higher than that of T1, T2 and T3 treatments in the two test areas, the other enzyme activities of T2 and T3 treatment were significantly higher than those of T1 treatment, which were significantly higher than those of CK treatment; in the combined application, except the urease activity of T2 treatment in Yong’an was the best but there was no significant difference from T3 treatment, the other enzyme activities of T3 treatment were higher than those of T2 treatment, indicating that biochar and cake fertilizer could improve soil enzyme activity; except the phosphatase activity of Jiangyong at 100 d after transplanting (late stage) was higher than that of Yong’an, the other three enzymes’ activities in Yong’an were significantly better than those in Jiangyong, showing the difference of soil texture (the former was red soil, the latter was sandy soil). In terms of the total amount of microorganism, T1 treatment was significantly higher than CK in Yong’an and Jiangyong. In terms of the total amount of bacteria of calcium cyanamide and its combined application with biochar and cake fertilizer, the order was T3>T2>T1>CK, in terms of the total amount of fungi, the order was T1>T3>T2>CK, in terms of the total amount of actinomycetes, the order was T1>T2>T3>CK, indicating that compared with the control, the application of calcium cyanamide could promote the increase of total microbial biomass; however, the combined application of calcium cyanamide with biochar and cake fertilizer could have different effects on the microbial biomass in tobacco planting soil: the application of calcium cyanamide combined with biochar and cake fertilizer could promote the growth of total bacteria, while the application of calcium cyanamide alone is beneficial to the increase of the total amount of fungi and actinomycetes.

Key words: tobacco planting soil, calcium cyanamide, soil amendments, biochar, cake fertilizer, soil urease, enzyme activity, microorganism

CLC Number:

S572

Dai Linjian, Chen Yu’an, Liu Chenxiang, Zhong Jun. Effects of Clcium Cyanamide and Its Combined Application with Biochar and Cake Fertilizer on Enzyme Activity and Total Microorganism Biomass in Tobacco Planting Soil[J]. Chinese Agricultural Science Bulletin, 2021, 37(34): 97-102.

Figures/Tables 7

References 30

[1]	刘思超, 唐利忠, 石泉, 等. 氰氨化钙在农业生产中的应用研究进展[J]. 中国土壤与肥料, 2017(5):1-6.
[2]	邬奇峰, 徐巧凤, 秦华, 等. 杀菌剂氰氨化钙对集约经营雷竹林土壤生物学性质的影响[J]. 浙江农林大学学报, 2014, 31(3):352-357.
[3]	马军伟, 孙万春, 胡庆发, 等. 氰胺类肥料对连作土壤微生物种群结构的影响[J]. 浙江大学学报:农业与生命科学版, 2013, 39(3):281-290.
[4]	王惠. 土壤改良剂对洛宁植烟土壤改良及烟草生长的影响[D]. 洛阳:河南科技大学, 2015.
[5]	袁永胜, 徐鲁政, 曹忠杰, 等. 氰氨化钙阿维菌素防治黄瓜根结线虫病田间药效试验[J]. 农业与技术, 2015, 35(10):161.
[6]	贲海燕. 氰氨化钙防治黄瓜根腐病及对土壤微生物的种群效应[D]. 沈阳:沈阳农业大学, 2014.
[7]	Tremblay N, Belec C, Coulombe J, et al. Evaluation of calcium cyanamide and liming for control of clubroot disease in cauliflower[J]. Crop Protection, 2005, 24:798-803. doi: 10.1016/j.cropro.2004.12.013 URL
[8]	Donald E C, Lawrence J M, Porter I J. Influence of particle size and application method on the efficacy of calcium cyanamide for control of clubroot of vegetable brassicas[J]. Crop Protection, 2004, 23:297-303. doi: 10.1016/j.cropro.2003.08.016 URL
[9]	常栋, 马文辉, 张凯, 等. 生物炭基肥对植烟土壤微生物功能多样性的影响[J]. 中国烟草学报, 2018, 24(6):58-66.
[10]	陈懿, 陈伟, 林叶春, 等. 生物炭对植烟土壤微生态和烤烟生理的影响[J]. 应用生态学报, 2015, 26(12):3781-3787.
[11]	王欣, 尹带霞, 张凤, 等. 生物炭对土壤肥力与环境质量的影响机制与风险解析[J]. 农业工程学报, 2015, 31(4):248-257.
[12]	陶朋闯, 陈效民, 靳泽文, 等. 生物质炭与氮肥配施对旱地红壤微生物量碳、氮和碳氮比的影响[J]. 水土保持学报, 2016, 30(1):231-235.
[13]	邓建强, 谭军, 施河丽, 等. 生物炭对土壤微生物调控效应[J]. 中国烟草学报, 2018, 24(3):46-52
[14]	李治玲. 生物炭对紫色土和黄壤养分、微生物及酶活性的影响[D]. 重庆:西南大学, 2016.
[15]	姚玲丹, 程广焕, 王丽晓, 等. 施用生物炭对土壤微生物的影响[J]. 环境化学, 2015, 34(4):69-74.
[16]	李影, 李斌, 姜桂英, 等. 植烟根际土壤生物活性对生物炭配施有机菌肥的响应[J]. 中国农学通报, 2019, 35(36):54-60.
[17]	王玉胜, 管成伟, 李旭华, 等. 饼肥配施对烤烟产质量与土壤改良的影响[J]. 广东农业科学, 2018, 45(3):45-50.
[18]	郭群召, 吴学巧, 黄平俊. 饼肥对土壤性状、烤烟生长及烟叶品质的影响[J]. 中国土壤与肥料, 2007(6):68-70.
[19]	李亮, 张佩佳, 张翔, 等. 不同饼肥配比对烟田土壤生物学特性及氮素转化的影响[J]. 土壤, 2019, 51(4):648-657.
[20]	靳志丽, 蒋尊龙, 贾少成, 等. 饼肥用量对湘南高有机质烟田烤烟生长及产质量的影响[J]. 作物研究, 2018, 32(6):516-520.
[21]	闫超, 刁晓林, 葛慧玲, 等. 水稻秸秆还田对土壤溶液养分与酶活性的影响[J]. 土壤通报, 2012, 43(5):1232-1236.
[22]	Mg V D H, Bardgett R D, van Straalen N M. The unseen majority: soil microbes as drivers of plant diversity and productivity in terrestrial ecosystems[J]. Ecology Letters, 2007, 11(3):296-310. doi: 10.1111/ele.2008.11.issue-3 URL
[23]	Harris J A. Measurements of the soil microbial community for estimating the success of restoration[J]. European Journal of Soil Science, 2010, 54(4):801-808. doi: 10.1046/j.1351-0754.2003.0559.x URL
[24]	林耀奔, 叶艳妹, 刘书畅, 等. 不同农地整治措施对土壤细菌多样性的影响[J]. 长江流域资源与环境, 2019, 28(11):2632-2640.
[25]	陈泽鑫, 曹帅婷. 土壤真菌在森林生态系统功能调控中的作用与意义[J]. 科学技术与创新, 2020, 12(5):155-157
[26]	薛文凯, 德吉, 朱攀, 等. 西藏墨脱土壤理化因子与放线菌物种多样性相关性分析[J]. 西藏科技, 2019, 12:24-28.
[27]	林书震, 李小龙, 李红丽, 等. 烟株生长过程中土壤微生物的变化特征[J]. 中国土壤与肥料, 2019, 5:14-24.
[28]	沈铮. 饼肥与营养液联用对烤烟根区生态环境和烟叶品质的影响[D]. 郑州:河南农业大学, 2010
[29]	和文祥, 朱铭莪. 陕西土壤腮酶活性与土壤肥力关系分析[J]. 土壤学报, 1997, 34(4):392-398.
[30]	李冰, 李玉双, 魏建兵, 等. 沈北新区不同土地利用类型土壤磷酸酶活性特征及其影响因素分析[J]. 生态科学, 2019, 38(4):48-55.