Effects of Effective Microorganisms on Growth Performance, Nutrient Digestibility and Fecal Ammonia Emission of BALB/C Mice

doi:10.11924/j.issn.1000-6850.casb2021-0148

Abstract

Abstract:

The purpose of this study is to determine the effects of dietary supplementation of effective microorganisms (EM) on growth performance, nutrient digestibility and fecal ammonia emission of BALB/C mice. A total of 120 BALB/C mice (4-week-old) with 60 males and 60 females were randomly divided into 4 groups with 6 replicates in each group (3 replicates for male and 3 replicates for female) and 5 mice in each replicate. EM added into diet of each group (groupⅠ,Ⅱ,Ⅲ, and Ⅳ) was 0, 200, 400 and 600 mg/kg, respectively. The experiment lasted for 7 weeks, including one week of adaption period and 6 weeks of experimental period. The results showed that dietary addition of 400 and 600 mg/kg EM significantly increased dry matter and ether extract digestibility of BALB/C mice (P<0.05), but had no significant effect on the digestibility of other nutrients (P>0.05). EM had no significant effect on the growth performance and fecal ammonia emission of BALB/C mice (P>0.05). It is concluded that dietary supplementation of EM could improve nutrient digestibility (dry matter and ether extract) of BALB/C mice and could not reduce ammonia emission, and the recommended supplementation is 400 mg EM per kilogram diet.

Key words: effective microorganisms, BALB/C mice, growth performance, nutrient digestibility, ammonia emission

CLC Number:

S865.1+3

CAO Qiumei, WANG Luyi, LI Xiaoman, LI Junda, LIU Mengtian, ZHENG Yao, WANG Lihua. Effects of Effective Microorganisms on Growth Performance, Nutrient Digestibility and Fecal Ammonia Emission of BALB/C Mice[J]. Chinese Agricultural Science Bulletin, 2022, 38(7): 124-128.

Figures/Tables 4

References 10

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饲粮组成		营养水平
原料	含量/%	成分	含量/%
玉米	30.00	粗蛋白质	19.30
面粉	20.00	粗脂肪	6.38
次粉	10.00	粗灰分	5.49
豆粕	28.36	钙	1.20
棉籽粕	3.60	总磷	0.78
菜籽粕	3.00	精氨酸	1.14
植物油	0.36	赖氨酸	1.40
鱼粉	0.96	蛋氨酸+胱氨酸	0.81
食盐	0.48	代谢能/(MJ/kg)	12.44
石粉	1.46
磷酸氢钙	0.78
预混料	1.00
合计	100.00

饲粮组成		营养水平
原料	含量/%	成分	含量/%
玉米	30.00	粗蛋白质	19.30
面粉	20.00	粗脂肪	6.38
次粉	10.00	粗灰分	5.49
豆粕	28.36	钙	1.20
棉籽粕	3.60	总磷	0.78
菜籽粕	3.00	精氨酸	1.14
植物油	0.36	赖氨酸	1.40
鱼粉	0.96	蛋氨酸+胱氨酸	0.81
食盐	0.48	代谢能/(MJ/kg)	12.44
石粉	1.46
磷酸氢钙	0.78
预混料	1.00
合计	100.00

生长性能	时期	组别					性别			P
生长性能	时期	Ⅰ	Ⅱ	Ⅲ	Ⅳ	SEM	雌鼠	雄鼠	SEM	P_EM	P_性别	P_EM×性别
平均体重/g	初重	11.01	11.05	10.97	11.00	0.09	10.60b	11.42a	0.06	0.94	<0.001	0.50
	Ⅰ期	19.09	18.66	19.04	18.71	0.24	17.46b	20.29a	0.17	0.49	<0.001	0.12
	Ⅱ期	20.20	19.76	20.10	19.80	0.24	18.09b	21.83a	0.17	0.50	<0.001	0.03
	Ⅲ期	21.36	21.09	21.46	21.03	0.25	19.26b	23.21a	0.18	0.59	<0.001	0.06
平均日增重/g	Ⅰ期	0.58	0.54	0.58	0.55	0.02	0.49b	0.63a	0.01	0.38	<0.001	0.13
	Ⅱ期	0.08	0.08	0.08	0.08	0.01	0.04b	0.11a	0.01	1.00	<0.001	0.43
	Ⅲ期	0.08	0.10	0.10	0.09	0.01	0.08	0.10	0.01	0.64	0.10	0.61
平均日采食量/g	Ⅰ期	3.53	3.43	3.18	3.27	0.13	3.12b	3.59a	0.09	0.25	0.002	0.24
	Ⅱ期	3.71	3.34	3.59	3.53	0.15	3.14b	3.95a	0.10	0.40	<0.001	0.15
	Ⅲ期	3.68b	3.86ab	4.21a	4.20a	0.14	3.71b	4.27a	0.10	0.046	0.001	0.13

生长性能	时期	组别					性别			P
生长性能	时期	Ⅰ	Ⅱ	Ⅲ	Ⅳ	SEM	雌鼠	雄鼠	SEM	P_EM	P_性别	P_EM×性别
平均体重/g	初重	11.01	11.05	10.97	11.00	0.09	10.60b	11.42a	0.06	0.94	<0.001	0.50
	Ⅰ期	19.09	18.66	19.04	18.71	0.24	17.46b	20.29a	0.17	0.49	<0.001	0.12
	Ⅱ期	20.20	19.76	20.10	19.80	0.24	18.09b	21.83a	0.17	0.50	<0.001	0.03
	Ⅲ期	21.36	21.09	21.46	21.03	0.25	19.26b	23.21a	0.18	0.59	<0.001	0.06
平均日增重/g	Ⅰ期	0.58	0.54	0.58	0.55	0.02	0.49b	0.63a	0.01	0.38	<0.001	0.13
	Ⅱ期	0.08	0.08	0.08	0.08	0.01	0.04b	0.11a	0.01	1.00	<0.001	0.43
	Ⅲ期	0.08	0.10	0.10	0.09	0.01	0.08	0.10	0.01	0.64	0.10	0.61
平均日采食量/g	Ⅰ期	3.53	3.43	3.18	3.27	0.13	3.12b	3.59a	0.09	0.25	0.002	0.24
	Ⅱ期	3.71	3.34	3.59	3.53	0.15	3.14b	3.95a	0.10	0.40	<0.001	0.15
	Ⅲ期	3.68b	3.86ab	4.21a	4.20a	0.14	3.71b	4.27a	0.10	0.046	0.001	0.13

养分	组别				SEM	P
养分	Ⅰ	Ⅱ	Ⅲ	Ⅳ	SEM	P
干物质	76.40b	77.20ab	80.57a	80.60a	0.99	0.01
粗蛋白质	81.04	81.59	83.59	83.23	1.02	0.25
粗脂肪	93.53ab	92.61b	94.47a	94.38a	0.49	0.047
粗灰分	47.36	46.14	51.48	50.82	2.34	0.32
钙	22.11	16.90	26.35	21.25	2.46	0.12
磷	37.48	34.02	41.14	39.68	2.98	0.38