Abstract: The aim is to investigate the effects of yeast culture on growth performance, immune function and intestinal flora of quails. 720 one-day-old healthy quails of Korean Dragon were chosen and randomly divided into four groups, including control group, test group I (basic diet + 0.1% yeast culture), test group II (basal diet + 0.15% yeast culture) and test group III (basic diet + 0.2% yeast culture), 6 replicates were set for each group and each repeat had 30 chickens, and the test period was 42 days. The results showed that group I, group Ⅱ and group Ⅲ could significantly increase ADG of quails and reduce the F/G of quails in 1-21 d, 22-42 d and 1-42 d, and the index of fabricius bursa in 21 d and 42 d quails and the indexes of thymus and spleen in 42 d quails significantly increased. All group Ⅰ, group Ⅱ and group Ⅲ could markedly decrease the numbers of Escherichia coli and Salmonella in cecum in 21 d quail, and group Ⅱ and group Ⅲ could obviously increase the number of Lactobacillus in cecum, and the difference between group Ⅰ and group Ⅲ was also significant. Group Ⅰ, group Ⅱ and group Ⅲ could also markedly decrease the numbers of Escherichia coli and Salmonella in cecum and obviously increase the number of Lactobacillus in 42 d quails, there was significant difference between group Ⅰand group Ⅲ. It indicated that the growth performance and immune function of quails could be increased and the structure of intestinal flora could be improved by feeding yeast culture. The appropriate dosage yeast culture was 0.15%.