Effects of Dietary Methionine and Cystine on Growth Performance, Methionine Metabolism and Protein Metabolism of Asian Red-tailed Catfish (Hemibagrus Wyckioides)

doi:10.11924/j.issn.1000-6850.casb2020-0244

Abstract

Abstract:

Previous experimental results showed that adding 0~1.5% methionine in the basic diet (0.5% methionine) had no significant effect on growth and biochemical indexes of Asian red-tailed catfish (Hemibagrus wyckioides), which is contrary to the traditional animal nutrition theory. Four experimental diets were formulated by adding crystal amino acid in the basic diet, including control diet (Con), methionine deficiency diet (-Met), cysteine deficiency diet (-Cys) and methionine and cysteine deficiency diet (-(Met+Cys)), and thereby to determine whether methionine is the essential amino acid for Hemibagrus wyckioides. The results showed that: The weight gain, feed intake and protein efficiency ratio of fish fed Con and -Cys diets were significantly higher than those of fish fed -Met and -(Met+Cys) diets. Conversely, the feed conversion rate was lower in fish fed Con and -Cys diets compared to fish fed -Met and -(Met+Cys) diets. The hepatic lipase activity of fish fed -Met and -(Met+Cys) diets was significantly lower than that of fish fed the Con diet. The disaccharidase activity was significantly higher in fish fed -Cys diet compared to that in fish fed Con diet. The hepatic AMPD activity of fish fed Con diet was significantly lower than that of fish fed -(Met+Cys) diet. Conversely, the alanine aminotransferase activity of fish fed -(Met+Cys) diet was significantly higher than that of other fish fed diets. The MAT and CBS activities of fish fed -Met diet were significantly higher than those of other fish fed diets. The BHMT activity of fish fed -(Met+Cys) diet was significantly higher than that of fish fed Con and -Cys diets. These results indicate that the lack of methionine could decrease the growth, protein metabolism and antioxidant capacity of Hemibagrus wyckioides. Therefore, methionine is the essential amino acid for Hemibagrus wyckioides.

Key words: Hemibagrus wyckioides, methionine, cysteine, growth performance, methionine metabolism, protein metabolism

CLC Number:

S963

Zhang Xindang, Li Guangbin, Wang Qiumei, Sun Yan, Huang Han, Su Linhai, Li Biwei, Deng Junming. Effects of Dietary Methionine and Cystine on Growth Performance, Methionine Metabolism and Protein Metabolism of Asian Red-tailed Catfish (Hemibagrus Wyckioides)[J]. Chinese Agricultural Science Bulletin, 2021, 37(20): 136-143.

Figures/Tables 8

References 39

[1]	周长海,贾友刚,齐淑艳,等.低鱼粉幼鲤饲料中添加DL-蛋氨酸及其羟基类似物的效果比较[J].科学养鱼,2011(4):65-66.
[2]	张卫东,张远方.饲料中添加蛋氨酸对鲤鱼生长性能的影响[J].河南水产,2004,16(4):29-30.
[3]	帅柯.蛋氨酸对幼建鲤消化功能和免疫功能的影响[D].雅安:四川农业大学,2006.
[4]	张满隆,邓理.蛋氨酸在鲫鱼饲料中的作用[J].饲料研究,2001,24(5):26-27.
[5]	Gjoen A U,Njaa L R.Methionine sulphoxide as a source of sulphur-containing amino acids for the young rat[J].British Journal of Nutrition,1977,37(1):93-105. pmid: 849403
[6]	Brosnan J T,Brosnan M E.Branched-Chain Amino Acids: Enzyme and Substrate Regulation[J].Journal of Nutrition,2006,136(1):207S-211S. doi: 10.1093/jn/136.1.207S URL
[7]	刘明典,陈大庆,段辛斌,等.澜沧江云南段鱼类区系组成与分布[J].中国水产科学,2011,18(1):156-170.
[8]	Ziv G,Baran E,Nam S,et al.Trading-off fish biodiversity, food security, and hydropower in the Mekong River Basin[J].Proceedings of the National Academy of Sciences of the United States of America,2012,109(15):5609-14.
[9]	阴双雨.澜沧江丝尾鳠生物学和资源[D].武汉:华中农业大学,2011.
[10]	陈云芬.土著丝尾鳠人工繁育获成功[N].云南日报,2010-04-06(002).
[11]	Deng J,Zhang X,Bi B,et al.Dietary protein requirement of juvenile Asian red-tailed catfish Hemibagrus wyckioides[J].Animal Feed Science & Technology,2011,170(3-4):231-238.
[12]	Deng J,Mai K,Chen L,et al.Effects of replacing soybean meal with rubber seed meal on growth, antioxidant capacity, non-specific immune response, and resistance to Aeromonas hydrophila, in tilapia (Oreochromis niloticus × O.aureus)[J].Fish & Shellfish Immunology,2015,44(2):436-444.
[13]	Mai K,Wan J,Ai Q,et al.Dietary methionine requirement of large yellow croaker, Pseudosciaena crocea R[J].Aquaculture,2006,251(2-4):346-353. doi: 10.1016/j.aquaculture.2005.05.038 URL
[14]	王蕾蕾,邵庆均.饲料中添加蛋氨酸对鱼类生长的影响[J].水利渔业,2007,27(1):7-10.
[15]	Shusaku T,Shimeno S,Hosokawa H,et al.Effect of lysine and methionine supplementation to a soy protein concentrate diet for red sea bream Pagrus major[J].Fisheries Science,2001,67(6):1088-1096. doi: 10.1046/j.1444-2906.2001.00365.x URL
[16]	魏玉婷.大菱鲆(Scophthalmus maximus)幼鱼对饲料中蛋氨酸、精氨酸、维生素A及维生素E需求量的研究[D].青岛:中国海洋大学,2010.
[17]	王香丽.蛋氨酸对瓦氏黄颡鱼幼鱼生长和代谢的影响[D].青岛:中国海洋大学,2014.
[18]	向枭,周兴华,罗莉,等.饲料蛋氨酸水平对吉富罗非鱼生长、饲料利用率和体成分的影响[J].水产学报,2014,38(4):538-549.
[19]	周贤君.异育银鲫对晶体赖氨酸和蛋氨酸的利用及需求量研究[D].武汉:华中农业大学,2005.
[20]	Fagbenro O A,Balogun A M,Fasakin E A.Dietary methionine requirement of the African catfish, Clarias gariepinus[J].Journal of Applied Aquaculture,1999,9(1):65-72. doi: 10.1300/J028v09n01_06 URL
[21]	Harding D E,Jr A O,Wilson R P.Sulfur amino acid requirement of channel catfish: L-methionine and L-cystine[J].Journal of Nutrition,1977,107(11):2031. pmid: 908960
[22]	Ahmed I.Dietary amino acid L-tryptophan requirement of fingerling Indian catfish, Heteropneustes fossilis (Bloch), estimated by growth and haemato-biochemical parameters[J].Aquaculture Research,2012,38(4):1195-1209.
[23]	唐炳荣.蛋氨酸对生长中期草鱼消化吸收能力和抗氧化能力影响的研究[D].雅安:四川农业大学,2012.
[24]	Houlihan D F,Hall S J,Gray C,et al.Growth Rates and Protein Turnover in Atlantic Cod, Gadus morhua[J].Canadian Journal of Fisheries & Aquatic Sciences,1990,45(6):951-964.
[25]	彭艳,唐凌,帅柯,等.蛋氨酸对幼建鲤生长及消化吸收功能的影响[J].中国畜牧杂志,2009,45(13):33-38.
[26]	Finkelstein J D.Methionine metabolism in mammals[J].Journal of Nutritional Biochemistry,1990.1(5):228-237. pmid: 15539209
[27]	Brosnan J T,Brosnan M E,Bertolo R F P,et al.Methionine: A metabolically unique amino acid[J].Livestock Science,2007,112(1-2):2-7. doi: 10.1016/j.livsci.2007.07.005 URL
[28]	Brosnan J T,Brosnan M E.The sulfur-containing amino acids: an overview[J].Journal of Nutrition,2006,136(6):1636S. doi: 10.1093/jn/136.6.1636S URL
[29]	计峰,武书庚,张海军,等.蛋氨酸来源调控机体蛋氨酸代谢的研究进展[J].中国畜牧杂志,2011,47(19):74-78.
[30]	Stipanuk M H.Sulfur amino acid metabolism: pathways for production and removal of homocysteine and cysteine[J].Annual Review of Nutrition,2004,24(1):539. doi: 10.1146/annurev.nutr.24.012003.132418 URL
[31]	赵珂立.氧化应激对动物自由基代谢、消化道损伤及抗氧化剂干预作用的研究[D].上海:上海交通大学,2011.
[32]	赖建东,魏日凤,连玲丽,等.炭疽病病原菌侵染对茶树叶片生理特性的影响[J].福建农林大学学报:自然版,2016,45(2):162-168.
[33]	徐晓琴.血清谷胱甘肽过氧化物酶比色测定法[J].南京医科大学学报:自然科学版,1998(4):330-331.
[34]	周小秋,杨凤,周安国,等.稚鳖蛋氨酸的营养需要量[J].水生生物学报,2003,27(1):69-73.
[35]	曾雯娉.凡纳滨对虾幼虾对赖氨酸、蛋氨酸、精氨酸和苯丙氨酸需要量的研究[D].湛江:广东海洋大学,2012.
[36]	Simmons L,Moccia R D,Bureau D P,et al.Dietary methionine requirement of juvenile Arctic charr Salvelinus alpinus (L.)[J].Aquaculture Nutrition,2015,5(2):93-100. doi: 10.1046/j.1365-2095.1999.00097.x URL
[37]	Cai Y,Burtle G J.Methionine requirement of channel catfish fed soybean meal-corn-based diets[J].Journal of Animal Science,1996,74(3):514-21. pmid: 8707704
[38]	Goff J B,Dmiii G.Evaluation of different sulfur amino acid compounds in the diet for red drum, Sciaenops ocellatus, and sparing value of cystine for methionine[J].Aquaculture,2004,241(1):465-477. doi: 10.1016/j.aquaculture.2004.08.002 URL
[39]	陈乃松,马建忠,周恒永,等.大口黑鲈对饲料中蛋氨酸需求量的评定[J].水产学报,2010,34(8):1244-1253.

			Con	-Met	-Cys	-(Met+Cys)
饲料配方		鱼粉	8.00	8.00	8.00	8.00
		豆粕	10.00	10.00	10.00	10.00
		明胶	9.00	9.00	9.00	9.00
		蛋氨酸	0.50	0.00	0.50	0.00
		天冬氨酸：谷氨酸=1:1	0.25	0.75	0.50	1.00
		半胱氨酸	0.25	0.25	0.00	0.00
		晶体氨基酸预混料¹	15.80	15.80	15.80	15.80
		羧甲基纤维素	3.00	3.00	3.00	3.00
		卡拉胶	2.00	2.00	2.00	2.00
		面粉	15.00	15.00	15.00	15.00
		α-淀粉	18.30	18.30	18.30	18.30
		鱼油	6.80	6.80	6.80	6.80
		豆油	7.30	7.30	7.30	7.30
		大豆卵磷脂	0.50	0.50	0.50	0.50
		Ca(H₂PO₄)₂	1.20	1.20	1.20	1.20
饲料配方		NaCl	0.30	0.30	0.30	0.30
		维生素C磷酸酯(35%)	0.03	0.03	0.03	0.03
		乙氧基喹啉(30%)	0.02	0.02	0.02	0.02
		氯化胆碱(50%)	0.25	0.25	0.25	0.25
		维生素预混料²	0.50	0.50	0.50	0.50
		矿物质预混料³	0.50	0.50	0.50	0.50
		牛磺酸	0.50	0.50	0.50	0.50
	营养组成	干物质(% DM)	90.97	92.33	91.58	92.06
		粗蛋白(% DM)	34.33	34.08	34.78	34.01
		粗脂肪(% DM)	21.88	15.93	16.07	16.07
		粗灰分(% DM)	6.15	6.59	6.37	6.55
		总能(MJ/kg DM)	22.08	21.79	21.77	21.96

			Con	-Met	-Cys	-(Met+Cys)
饲料配方		鱼粉	8.00	8.00	8.00	8.00
		豆粕	10.00	10.00	10.00	10.00
		明胶	9.00	9.00	9.00	9.00
		蛋氨酸	0.50	0.00	0.50	0.00
		天冬氨酸：谷氨酸=1:1	0.25	0.75	0.50	1.00
		半胱氨酸	0.25	0.25	0.00	0.00
		晶体氨基酸预混料¹	15.80	15.80	15.80	15.80
		羧甲基纤维素	3.00	3.00	3.00	3.00
		卡拉胶	2.00	2.00	2.00	2.00
		面粉	15.00	15.00	15.00	15.00
		α-淀粉	18.30	18.30	18.30	18.30
		鱼油	6.80	6.80	6.80	6.80
		豆油	7.30	7.30	7.30	7.30
		大豆卵磷脂	0.50	0.50	0.50	0.50
		Ca(H₂PO₄)₂	1.20	1.20	1.20	1.20
饲料配方		NaCl	0.30	0.30	0.30	0.30
		维生素C磷酸酯(35%)	0.03	0.03	0.03	0.03
		乙氧基喹啉(30%)	0.02	0.02	0.02	0.02
		氯化胆碱(50%)	0.25	0.25	0.25	0.25
		维生素预混料²	0.50	0.50	0.50	0.50
		矿物质预混料³	0.50	0.50	0.50	0.50
		牛磺酸	0.50	0.50	0.50	0.50
	营养组成	干物质(% DM)	90.97	92.33	91.58	92.06
		粗蛋白(% DM)	34.33	34.08	34.78	34.01
		粗脂肪(% DM)	21.88	15.93	16.07	16.07
		粗灰分(% DM)	6.15	6.59	6.37	6.55
		总能(MJ/kg DM)	22.08	21.79	21.77	21.96

氨基酸	Con	-Met	-Cys	-(Met+Cys)
天冬氨酸Asp	4.40	4.47	4.44	4.30
谷氨酸Glu	7.43	7.20	7.48	7.45
丝氨酸Ser	1.48	1.50	1.41	1.45
甘氨酸Gly	3.98	3.80	3.75	3.52
组氨酸His	0.72	0.75	0.70	0.71
精氨酸Arg	4.51	4.65	4.45	4.47
苏氨酸Thr	1.91	1.84	1.79	1.77
丙氨酸Ala	2.94	2.96	2.87	2.85
脯氨酸Pro	3.57	3.37	3.36	3.49
酪氨酸Tyr	0.87	0.92	0.89	0.86
缬氨酸Val	1.75	1.83	1.77	1.79
蛋氨酸Met	0.71	0.20	0.73	0.22
半胱氨酸Cys	0.33	0.34	0.10	0.09
异亮氨酸Ile	2.01	2.08	1.94	1.97
亮氨酸Leu	3.11	3.24	3.04	2.98
苯丙氨酸Phe	1.87	1.89	1.83	1.85
赖氨酸Lys	5.70	5.60	5.55	5.53

氨基酸	Con	-Met	-Cys	-(Met+Cys)
天冬氨酸Asp	4.40	4.47	4.44	4.30
谷氨酸Glu	7.43	7.20	7.48	7.45
丝氨酸Ser	1.48	1.50	1.41	1.45
甘氨酸Gly	3.98	3.80	3.75	3.52
组氨酸His	0.72	0.75	0.70	0.71
精氨酸Arg	4.51	4.65	4.45	4.47
苏氨酸Thr	1.91	1.84	1.79	1.77
丙氨酸Ala	2.94	2.96	2.87	2.85
脯氨酸Pro	3.57	3.37	3.36	3.49
酪氨酸Tyr	0.87	0.92	0.89	0.86
缬氨酸Val	1.75	1.83	1.77	1.79
蛋氨酸Met	0.71	0.20	0.73	0.22
半胱氨酸Cys	0.33	0.34	0.10	0.09
异亮氨酸Ile	2.01	2.08	1.94	1.97
亮氨酸Leu	3.11	3.24	3.04	2.98
苯丙氨酸Phe	1.87	1.89	1.83	1.85
赖氨酸Lys	5.70	5.60	5.55	5.53

项目	Con	-Met	-Cys	-(Met+Cys)
初重/g	2.70 ± 0.00	2.71 ± 0.01	2.71 ± 0.01	2.70 ± 0.01
末重/g	12.17 ± 0.13^b	5.93 ± 0.10^a	11.48 ± 0.54^b	5.37 ± 0.08^a
增重率/%	3.51 ± 0.05^b	1.19 ± 0.03^a	3.24 ± 0.21^b	0.98 ± 0.03^a
日增重系数/(%/d)	1.46 ± 0.01^b	0.67 ± 0.01^a	1.39 ± 0.06^b	0.58 ± 0.01^a
总摄食量/(g/fish)	13.20 ± 0.44^b	8.02 ± 0.23^a	12.94 ± 0.56^b	6.38 ± 0.20^a
摄食率/(%/MBW/d)	8.78 ± 0.24^b	7.78 ± 0.27^a	8.89 ± 0.23^b	6.49 ± 0.16^a
饲料系数	1.40 ± 0.03^a	2.49 ± 0.13^b	1.48 ± 0.05^a	2.40 ± 0.04^b
蛋白质效率	2.09 ± 0.04^b	1.19 ± 0.06^a	1.95 ± 0.06^b	1.23 ± 0.02^a
存活率/%	96.67 ± 0.83	99.17 ± 0.83	98.33 ± 0.83	99.17 ± 0.83