Genetic Diversity and Genetic Structure of Coilia nasustaihuensis Population Based on Cyt b Gene in Jiangsu Province

doi:10.11924/j.issn.1000-6850.casb2020-0336

Abstract

Abstract:

To scientifically manage and rationally use the germplam resources of Coilia nasustaihuensis, it is necessary to understand its background. In the present study, the mitochondrial DNA cytochrome b (Cyt b) genes were amplified and sequenced from 224 C. nasustaihuensis specimens that were collected from six freshwater lakes (Lake Tai, Lake Ge, Lake Gaoyou, Lake Baima, Lake Hongze, Lake Luoma). The results showed that 62 polymorphic nucleotide sites and 57 haplotypes were detected among the Cyt b sequences of 224 individuals. The haplotype diversity (h) and nucleotide diversity (π) were 0.783±0.028 and 0.00260±0.00019, respectively. AMOVA analysis indicated that genetic variations between the populations from different lakes were mainly within the populations. Genetic differentiation index (F_st) suggested high genetic differentiation between Lake Ge population and the other five populations. The results of neutrality test showed that the values of Tajima’s D and Fu’s F_s were significant negative, and the mismatch distribution analysis map showed a peak type (except Lake Ge population), indicating that the other lake populations had undergone population expansion in evolutionary history, and the population expansion appeared in 0.12 Mya. The research indicates that the genetic diversity of C. nasustaihuensis of six lakes’ populations is abundant in Jiangsu province. The lake Ge population owns multiple specific haplotypes, and there are high genetic differences between Lake Ge population and the other populations. Therefore, the Lake Ge population and the populations of other five lakes should be managed and utilized as distinct evolutionarily significant units.

Key words: Coilia nasustaihuensis, Cyt b gene, genetic diversity, genetic structure, population historical dynamics

CLC Number:

S917.4

Li Daming, Tang Shengkai, Liu Yanshan, Gu Xiankun, Yin Jiawen, Jiang Qichen, Zhang Tongqing, Pan Jianlin. Genetic Diversity and Genetic Structure of Coilia nasustaihuensis Population Based on Cyt b Gene in Jiangsu Province[J]. Chinese Agricultural Science Bulletin, 2021, 37(20): 144-151.

Figures/Tables 8

References 30

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群体	采样时间	经纬度	样品数量
滆湖	2018年10月	31.6755ºN, 119.8444ºE	31
太湖	2018年10月	31.3061ºN, 120.1309ºE	39
高邮湖	2016年12月	32.8381ºN, 119.3414ºE	38
白马湖	2017年4月	33.2829ºN, 119.1379ºE	40
洪泽湖	2016年12月	33.3264ºN, 118.7518ºE	40
骆马湖	2017年4月	34.1443ºN, 118.2253ºE	36

群体	采样时间	经纬度	样品数量
滆湖	2018年10月	31.6755ºN, 119.8444ºE	31
太湖	2018年10月	31.3061ºN, 120.1309ºE	39
高邮湖	2016年12月	32.8381ºN, 119.3414ºE	38
白马湖	2017年4月	33.2829ºN, 119.1379ºE	40
洪泽湖	2016年12月	33.3264ºN, 118.7518ºE	40
骆马湖	2017年4月	34.1443ºN, 118.2253ºE	36

群体	样品数	变异位点	单倍型（个体数）	单倍型多样性	核苷酸多样性
滆湖	31	24	H3(1), H11(2), H19(9), H20(1), H21(1), H22(1),H23(1), H24(1), H25(1), H41(1), H42(1),H43(1), H44(1), H45(1), H49(7), H52(1)	0.875±0.044	0.00425±0.00033
太湖	39	24	H1(3), H9(1), H10(2), H12(1), H14(1), H31(1),H33(1), H34(1), H35(1), H36(1), H37(1), H39(1),H40(1), H49(19), H50(1), H53(1), H54(1)	0.762±0.073	0.00210±0.00030
高邮湖	38	26	H4(4), H8(1), H11(1), H12(1), H13(1), H18(1),H26(3), H27(1), H38(2), H43(1), H48(1),H49(20), H56(1), H57(1)	0.718±0.078	0.00270±0.00057
白马湖	40	17	H2(2), H4(3), H6(2), H15(1), H16(1), H18(1),H126(2), H29(1), H38(5), H47(1), H49(19),H55(1)	0.760±0.067	0.00216±0.00036
洪泽湖	40	14	H2(1), H4(4), H5(1), H7(1), H16(2), H18(1),H28(2), H30(1), H32(1), H38(4), H46(2),H49(19), H51(1)	0.762±0.067	0.00182±0.00028
骆马湖	36	13	H4(9), H17(1), H26(3), H38(3), H46(2), H47(1),H48(1), H49(17)	0.717±0.060	0.00180±0.00034
合计	224	62		0.783±0.028	0.00260±0.00019

群体	样品数	变异位点	单倍型（个体数）	单倍型多样性	核苷酸多样性
滆湖	31	24	H3(1), H11(2), H19(9), H20(1), H21(1), H22(1),H23(1), H24(1), H25(1), H41(1), H42(1),H43(1), H44(1), H45(1), H49(7), H52(1)	0.875±0.044	0.00425±0.00033
太湖	39	24	H1(3), H9(1), H10(2), H12(1), H14(1), H31(1),H33(1), H34(1), H35(1), H36(1), H37(1), H39(1),H40(1), H49(19), H50(1), H53(1), H54(1)	0.762±0.073	0.00210±0.00030
高邮湖	38	26	H4(4), H8(1), H11(1), H12(1), H13(1), H18(1),H26(3), H27(1), H38(2), H43(1), H48(1),H49(20), H56(1), H57(1)	0.718±0.078	0.00270±0.00057
白马湖	40	17	H2(2), H4(3), H6(2), H15(1), H16(1), H18(1),H126(2), H29(1), H38(5), H47(1), H49(19),H55(1)	0.760±0.067	0.00216±0.00036
洪泽湖	40	14	H2(1), H4(4), H5(1), H7(1), H16(2), H18(1),H28(2), H30(1), H32(1), H38(4), H46(2),H49(19), H51(1)	0.762±0.067	0.00182±0.00028
骆马湖	36	13	H4(9), H17(1), H26(3), H38(3), H46(2), H47(1),H48(1), H49(17)	0.717±0.060	0.00180±0.00034
合计	224	62		0.783±0.028	0.00260±0.00019

群体	滆湖	太湖	高邮湖	白马湖	洪泽湖	骆马湖
滆湖		0.24852^**	0.17516^**	0.19904^**	0.22608^**	0.19278^**
太湖	0.00418		0.00721	0.00798	0.00192	0.01533
高邮湖	0.00419	0.00242		-0.01096	-0.00957	-0.01339
白马湖	0.00396	0.00215	0.00240		-0.01588	-0.01213
洪泽湖	0.00387	0.00196	0.00223	0.00196		-0.01425
骆马湖	0.00372	0.00198	0.00222	0.00196	0.00178