Culter alburnus Basilewsky in Different Populations: Genetic Diversity Analysis Based on Cytochrome B (Cyt b) Gene

doi:10.11924/j.issn.1000-6850.casb2021-0127

Abstract

Abstract:

In order to understand the genetic diversity of different Culter alburnus Basilewsky populations in the lower reaches of the Changjiang River, and evaluate the resource status of C. alburnus. A total of 211 C. alburnus samples from Dianshan Lake (DSH), Taihu Lake (TH), Changdang Lake (CD) and Changjiang River (CJ) were studied. Mitochondrial DNA b gene (Cyt b) of C. alburnus was sequenced, and compared with NCBI. Genetic diversity data of four C. alburnus populations were analyzed by MEGA, DNASP and other software. The Cyt b gene sequence was 1088 bp in length, with 55% A+T and 45% G+C. A total of 258 polymorphic sites were detected, accounting for 23.71% of the total nucleotides. 46 haplotypes were found in 211 samples from 4 populations, and the haplotype diversity ranged from 0.849 to 0.946, the order was as following: TH>CD>CJ>DSH. The nucleotide diversity was 0.0083-0.174, which was represented as DSH> CD>CJ>DSH. The genetic distance between the four populations was 0.052-0.152, and the maximum was 0.152 between Taihu population and Dianshan Lake population. The genetic differentiation indices ranged from 0.197 to 0.646, and the gene flow values ranged from 0.152 to 1.019, among which the gene flow values of Changjiang River and Dianshan Lake populations were the smallest, while those of Changjiang River and Changdang Lake populations were the largest. The results of phylogenetic tree based on NJ method showed that there was no obvious geographical differentiation among the four populations. The results of this study indicated that the population genetic diversity of C. alburnus in the lower reaches of the Changjiang River was high. Combined with the results of fishery resources investigation in recent years, the genetic background and germplasm resources of C. alburnus in the lower reaches of the Changjiang were further understood, which provided basic data for wild resource conservation.

Key words: Culter alburnus Basilewsky, mitochondrial DNA, Cytochrome b gene, genetic diversity

CLC Number:

S932.4

Zhang Guining, Fang Di’an, Xue Xiangping, Zhang Minying, Feng Xiaoting, Yang Xuejun. Culter alburnus Basilewsky in Different Populations: Genetic Diversity Analysis Based on Cytochrome B (Cyt b) Gene[J]. Chinese Agricultural Science Bulletin, 2021, 37(23): 118-124.

Figures/Tables 7

References 31

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群体	序列数	单倍型数	Hd	K	Pi	Tajima's D	FU’s-test
TH	40	9	0.946±0.015	5.860	0.0083	-2.246^**	-1.137
DSH	47	17	0.849±0.004	104.463	0.174	-1.413	1.398
CD	40	8	0.944±0.017	32.687	0.035	0.969	-2.559^*
CJ	84	17	0.890±0.019	21.582	0.026	-2.859^**	1.967^*
总和	211	46	0.9035	38.254	0.0608	0.202	0.310

群体	序列数	单倍型数	Hd	K	Pi	Tajima's D	FU’s-test
TH	40	9	0.946±0.015	5.860	0.0083	-2.246^**	-1.137
DSH	47	17	0.849±0.004	104.463	0.174	-1.413	1.398
CD	40	8	0.944±0.017	32.687	0.035	0.969	-2.559^*
CJ	84	17	0.890±0.019	21.582	0.026	-2.859^**	1.967^*
总和	211	46	0.9035	38.254	0.0608	0.202	0.310

群体 1	群体2	Hs	Kxy	Gst	Dxy	Da
CD	CJ	0.75	98.67	0.03	0.40	0.08
CD	DSH	0.80	179.46	0.11	0.73	0.30
CD	TH	0.67	184.44	0.20	0.75	0.48
CJ	DSH	0.82	179.65	0.08	0.73	0.18
CJ	TH	0.73	185.67	0.15	0.75	0.37
DSH	TH	0.79	183.38	0.12	0.75	0.25

群体 1	群体2	Hs	Kxy	Gst	Dxy	Da
CD	CJ	0.75	98.67	0.03	0.40	0.08
CD	DSH	0.80	179.46	0.11	0.73	0.30
CD	TH	0.67	184.44	0.20	0.75	0.48
CJ	DSH	0.82	179.65	0.08	0.73	0.18
CJ	TH	0.73	185.67	0.15	0.75	0.37
DSH	TH	0.79	183.38	0.12	0.75	0.25