Genetic Diversity of Pseudomonas syringae pv. actinidiae strains in Sichuan

doi:10.11924/j.issn.1000-6850.casb18110082

Abstract

Abstract:

To determine the genetic diversity of kiwifruit ulcer pathogen Pseudomonas syringae pv. actinidiae (Psa) in Sichuan and the correlation between genetic group division and geographical origin, 40 Psa strains from different regions of Sichuan were used as the research materials, rep-PCR was used for molecular marker, and UPGMA clustering was conducted with NTSYS software. A total of 42 loci were obtained by 3 primer combinations (REP, ERIC and BOX), including 38 polymorphic loci, the rate of polymorphism was 90.5%. The results of UPMGA cluster analysis indicated that 40 Psa strains were divided into two groups (Ⅰ, Ⅱ) in the genetic similarity coefficient of 0.70, and 85.0% of the strains belonged to the Ⅱ group. The group Ⅰ contained two sub-groups (I-1, I-2) and the group Ⅱ consisted of five sub-groups (Ⅱ-1, Ⅱ-2, Ⅱ-3, Ⅱ-4, Ⅱ-5) in the genetic similarity coefficient of 0.80, but all strains had no obvious clustering of collecting locations. This study showed a rich polymorphism of Psa strains from different regions of Sichuan, but the genetic diversity had no significant correlation with geographical location.

Key words: kiwifruit, Pseudomonas syringae pv. actinidiae, rep-PCR, genetic diversity, genetic variation

CLC Number:

S432.1

Xiaoxiang Yang, Wenxian Wu, Xiaoqin Huang, Lei Zhang, Lei Du, Yong Liu. Genetic Diversity of Pseudomonas syringae pv. actinidiae strains in Sichuan[J]. Chinese Agricultural Science Bulletin, 2020, 36(9): 132-137.

Figures/Tables 6

References 22

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编号	名称	采集地	编号	名称	采集地	编号	名称	采集地
1	CX1	苍溪	15	QL3	邛崃	29	PZ2	彭州
2	CX2	苍溪	16	QL4	邛崃	30	PZ3	彭州
3	CX3	苍溪	17	PJ1	蒲江	31	PZ4	彭州
4	CX4	苍溪	18	PJ2	蒲江	32	PZ5	彭州
5	CX5	苍溪	19	PJ3	蒲江	33	PZ6	彭州
6	JY1	江油	20	PJ4	蒲江	34	PZ8	彭州
7	JY2	江油	21	PJ5	蒲江	35	PZ8	彭州
8	MN1	冕宁	22	PJ6	蒲江	36	SF1	什邡
9	MN2	冕宁	23	DJY1	都江堰	37	SF2	什邡
10	MS1	名山	24	DJY2	都江堰	38	SF3	什邡
11	MS2	名山	25	DJY3	都江堰	39	SF4	什邡
12	MS3	名山	26	DJY4	都江堰	40	SF5	什邡
13	QL1	邛崃	27	DJY5	都江堰
14	QL2	邛崃	28	PZ1	彭州

编号	名称	采集地	编号	名称	采集地	编号	名称	采集地
1	CX1	苍溪	15	QL3	邛崃	29	PZ2	彭州
2	CX2	苍溪	16	QL4	邛崃	30	PZ3	彭州
3	CX3	苍溪	17	PJ1	蒲江	31	PZ4	彭州
4	CX4	苍溪	18	PJ2	蒲江	32	PZ5	彭州
5	CX5	苍溪	19	PJ3	蒲江	33	PZ6	彭州
6	JY1	江油	20	PJ4	蒲江	34	PZ8	彭州
7	JY2	江油	21	PJ5	蒲江	35	PZ8	彭州
8	MN1	冕宁	22	PJ6	蒲江	36	SF1	什邡
9	MN2	冕宁	23	DJY1	都江堰	37	SF2	什邡
10	MS1	名山	24	DJY2	都江堰	38	SF3	什邡
11	MS2	名山	25	DJY3	都江堰	39	SF4	什邡
12	MS3	名山	26	DJY4	都江堰	40	SF5	什邡
13	QL1	邛崃	27	DJY5	都江堰
14	QL2	邛崃	28	PZ1	彭州

引物名称	引物序列(5'--3')
REP1R-I	IIIICGICGICATCIGGC
REP2-I	ICGICTTATCIGGCCTAC
ERIC1R	ATGTAAGCTCCTGGGGATTCAC
ERIC2	AAGTAAGTGACTGGGGTGAGCG
BOXA1R	CTACGGCAAGGCGACGCTGACG
PsaF1	TTTTGCTTTGCACACCCGATTTT
PsaR2	CACGCACCCTTCAATCAGGATG

引物名称	引物序列(5'--3')
REP1R-I	IIIICGICGICATCIGGC
REP2-I	ICGICTTATCIGGCCTAC
ERIC1R	ATGTAAGCTCCTGGGGATTCAC
ERIC2	AAGTAAGTGACTGGGGTGAGCG
BOXA1R	CTACGGCAAGGCGACGCTGACG
PsaF1	TTTTGCTTTGCACACCCGATTTT
PsaR2	CACGCACCCTTCAATCAGGATG

引物	位点总数	多态性位点数	多态性比率/%
REP1R-I/REP2-I	10	9	90.0
ERIC1/ERIC2	16	15	93.8
BOXA1R	16	14	87.5
总数	42	38	90.5
平均数	14	12.7	90.5