Abstract:

In order to study the genetic basis and genetic relationship of current rice varieties in Heilongjiang Province, 47 pairs of SSR markers published in the agricultural industry standard Protocol for the Identification of Rice Varieties SSR Marker Method (NY/T 1433—2014) were used to analyze the genetic diversity among 231 rice varieties from different accumulated temperature regions in Heilongjiang Province. The results showed that a total of 136 alleles were detected form the 47 pairs of SSR markers, alleles per locus ranged from 2 to 5, the average was 2.92. Genetic diversity ranged from 0.11 to 0.79, with an average of 0.56. The polymorphic information content (PIC) was 0.11-0.76, with an average of 0.49. The value of marker index (MI) ranged from 3.18 to 18.39, with an average of 6.52. Cluster analysis divided the 231 rice varieties into 3 categories and 7 groups, and the clustering results were consistent with the results of principal component analysis. Taken together, the genetic diversity of rice varieties in Heilongjiang Province is not rich enough (the average polymorphic information content index is 0.49), and the genetic relationship among the varieties in the same accumulated temperature area is relatively close. In variety breeding, attention should be paid to the hybridization of northern and southern varieties, in order to broaden the genetic background and cultivate new environment-friendly varieties with high-quality and high-yield.

Key words: Heilongjiang Province, rice varieties, SSR markers, genetic diversity, cluster analysis