5 Polygonum tinctorium Ait. Populations: Genetic Diversity Analysis Based on ISSR Marker

doi:10.11924/j.issn.1000-6850.casb2020-0765

Abstract

Abstract:

The research aims at analyzing genetic diversity and relationship of 5 Polygonum tinctorium Ait. populations, and providing molecular references to the germplasm resources protection, exploitation and utilization of P. tinctorium Ait.. 5 P. tinctorium Ait. populations were used as materials. The genetic diversity was studied by simple inter-sequence repeat (ISSR) makers. The software Popgene 32 was used to analyze the amplification results, and the NTsys2.10e was used to construct a cluster map according to the unweighted pair-group method with arithmetic means (UPGMA). The results showed that a total of 155 loci were amplified by 23 primers, of which 148 were polymorphic loci; the Nei's genetic diversity coefficient (H) was 0.3538, Shannon's Information index (I) was 0.5234, the coefficient of genetic differentiation (Gst) was 0.4096, and the gene flow (Nm) was 0.7206. Taking the genetic distance 0.234 as a threshold, the 5 populations were divided into three groups, among which the Japanese population was different from others. The results indicate that the genetic difference among the populations of P. tinctorium Ait. is related to the geographical distance. This study could provide a theoretical basis for the genetic diversity of P. tinctorium Ait. and the protection and utilization of germplasm resources.

Key words: Polygonum tinctorium Ait., ISSR molecular marker, genetic diversity, cluster analysis

CLC Number:

Xu Ming, Wang Qiong, Li Dongling, Zheng Yuhong, Xu Zenglai. 5 Polygonum tinctorium Ait. Populations: Genetic Diversity Analysis Based on ISSR Marker[J]. Chinese Agricultural Science Bulletin, 2021, 37(21): 134-139.

Figures/Tables 6

References 27

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序号	编号	采集地	样本量
1	SC	四川省凉山彝族自治州布拖县	5
2	SD	山东省日照市	5
3	JS	江苏省南通港闸区	5
4	GZ	贵州省黎平县	5
5	RB	日本德岛县（引种于本园）	5

序号	编号	采集地	样本量
1	SC	四川省凉山彝族自治州布拖县	5
2	SD	山东省日照市	5
3	JS	江苏省南通港闸区	5
4	GZ	贵州省黎平县	5
5	RB	日本德岛县（引种于本园）	5

引物名称	序列(5'-3')	总位点数	多态性位点数	多态性位点百分率/%
801	(AT)8T	4	4	100
803	(AT)8C	8	8	100
804	(TA)8A	6	5	83.33
805	(TA)8C	8	8	100
807	(AG)8T	8	7	87.5
808	(AG)8C	8	7	87.5
810	(GA)8T	6	6	100
811	(GA)8C	7	7	100
812	(GA)8A	6	5	83.33
814	(CT)8A	4	3	75
815	(CT)8G	5	5	100
817	(CA)8A	8	8	100
823	(TC)8C	4	4	100
832	(AT)8CTC	7	7	100
836	(AG)8CTA	6	5	83.33
842	(GA)8CTG	8	8	100
843	(CT)8AGA	8	8	100
844	(CT)8AGC	6	6	100
845	(CT)8AGG	8	8	100
847	(CA)8AGC	8	8	100
857	(AC)8CTG	9	8	88.89
859	(TG)8AGC	8	8	100
874	(CCCT)4	5	5	100
平均	--	6.74	6.43	95.48
总数	--	155	148	--

引物名称	序列(5'-3')	总位点数	多态性位点数	多态性位点百分率/%
801	(AT)8T	4	4	100
803	(AT)8C	8	8	100
804	(TA)8A	6	5	83.33
805	(TA)8C	8	8	100
807	(AG)8T	8	7	87.5
808	(AG)8C	8	7	87.5
810	(GA)8T	6	6	100
811	(GA)8C	7	7	100
812	(GA)8A	6	5	83.33
814	(CT)8A	4	3	75
815	(CT)8G	5	5	100
817	(CA)8A	8	8	100
823	(TC)8C	4	4	100
832	(AT)8CTC	7	7	100
836	(AG)8CTA	6	5	83.33
842	(GA)8CTG	8	8	100
843	(CT)8AGA	8	8	100
844	(CT)8AGC	6	6	100
845	(CT)8AGG	8	8	100
847	(CA)8AGC	8	8	100
857	(AC)8CTG	9	8	88.89
859	(TG)8AGC	8	8	100
874	(CCCT)4	5	5	100
平均	--	6.74	6.43	95.48
总数	--	155	148	--

居群	多态性位点数	多态性位点百分率/%	Na	Ne	H	I
居群	多态性位点数	多态性位点百分率/%	均值	均值	均值	均值
SC	72	46.45	1.4645±0.5004	1.3147±0.3902	0.1787±0.2079	0.2629±0.2972
SD	82	52.90	1.5290±0.5008	1.3461±0.3924	0.1978±0.2071	0.2927±0.2952
JS	73	47.10	1.4710±0.5008	1.3242±0.4008	0.1821±0.2107	0.2672±0.2997
GZ	95	61.29	1.6129±0.4887	1.4780±0.4333	0.2591±0.2224	0.3729±0.3118
RB	86	55.48	1.5548±0.4986	1.4049±0.4080	0.2265±0.2158	0.3295±0.3070
居群水平			1.5264±0.4979	1.3736±0.4049	0.2088±0.2128	0.3050±0.3022
物种水平			1.9548±0.2083	1.6161±0.3147	0.3538±0.1446	0.5234±0.1856