Abstract:

To provide theoretical reference for classification and identification of Pleurotus pulmonarius germplasm resources and genetic breeding, the genetic diversity and agronomic traits of P. pulmonarius strains were analyzed. Antagonistic reaction, ISSR molecular marker technology and agronomic trait evaluation were used to investigate the genetic diversity of 10 P. pulmonarius strains with high yields. The results showed that the genetic similarity level of the 10 tested P. pulmonarius strains ranged from 0.63 to 0.92. Among them, the genetic similarity coefficients of PL7 and PL8 were the highest, indicating that the relationship between the two was the closest; the genetic similarity coefficients among the remaining strains were low, and the genetic similarity was relatively low. When the similarity coefficient was 0.69, the 10 P. pulmonarius strains were divided into three major groups; the yield of the tested strains on the substrate of apple sawdust and corncob was relatively high, and the biological conversion rate was 75.64%-88.21%. There were big differences in terms of the agronomic traits, the cap color included six types: yellow-white, gray, white and brown, light yellow, brown, and dark gray; and the stalks were divided into three types: slender, medium thick, and thick and long. Antagonistic reaction, ISSR molecular marker technology and agronomic trait evaluation can accurately identify the genetic differences of the tested P. pulmonarius strains, and the results show that the 10 tested strains have rich genetic differences and genetic diversities.

Key words: Pleurotus pulmonarius, antagonistic reaction, molecular marker, genetic similarity coefficient, genetic diversity, agronomic traits