Identification of the Pathogen of Ficus carica Epidemic Disease in Sichuan

doi:10.11924/j.issn.1000-6850.casb20200100055

Abstract

Abstract:

To identify the pathogen species of Ficus carica epidemic disease outbreak in Sichuan Province, the occurrence of F. carica epidemic disease in F. carica planting areas in Sichuan was investigated, and the diseased fruits were collected. The pathogen was isolated and cultured by tissue isolation method, and was identified by combining morphology and molecular identification. The results showed that the pathogen mycelium was white, the oospores were spherical, and the sporangiums were oval or ovoid, with papillae at the top. The sequence analysis revealed 99% sequence identities with Phytophthora palmivora. According to morphology and molecular identification, the pathogen of F. carica epidemic disease in Sichuan Province was identified as P. palmivora.

Key words: Ficus carica, phytophthora blight, pathogenic fungi, isolation and identification, Phytophthora palmivora

CLC Number:

S432.1

Zhai Min, Xian Yunxi, Huang Xiaoqin, Zhang Lei, Zhou Xiquan, Liu Yong, Yang Xiaoxiang. Identification of the Pathogen of Ficus carica Epidemic Disease in Sichuan[J]. Chinese Agricultural Science Bulletin, 2020, 36(17): 123-128.

Figures/Tables 5

References 35

[1]	Amani Shahla, Mohebodini Mehdi, Khademvatan Shahram , et al. Agrobacterium rhizogenes mediated transformation of Ficus carica L. for the efficient production of secondary metabolites[J]. Journal of thescience of food and agriculture, 2020.
[2]	汪莉莉, 付冰, 李小凤 , 等. 无花果果糕的研制及其质构特性[J]. 食品工业科技, 2018,22:163-168.
[3]	王玉玲, 潘一山, 李晔 . 波姬红无花果果干制作方法筛选[J]. 福建热作科技, 2017(1):1-5.
[4]	隆旺夫 . 无花果果脯、果酱的加工[J]. 山西果树, 2006,5:51-52.
[5]	张倩, 胡金涛, 王晓芳 , 等. 无花果果脯加工工艺研究[J]. 落叶果树, 2016(5):46-47.
[6]	任雪婷, 李春英, 赵春健 , 等. 无花果加工利用研究现状[J]. 黑龙江农业科学, 2019(5):156-158.
[7]	白玉梅, 徐超, 杨鹤同 . 无花果疫霉果腐病[J]. 林业科技开发, 2014(3):134-136.
[8]	黄海 . 无花果疫腐病及其防治[J]. 江西果树, 1997(2):29.
[9]	褚方钢 . 无花果三大病害的发生及防治[J]. 烟台果树, 2011(4):53-54.
[10]	张小燕, 李国栋, 张建国 , 等. 无花果病虫害鉴别及防治[J]. 中国果蔬, 2016(4):45-47.
[11]	王永明 . 无花果病虫害及其防治[J]. 安徽农业科技, 2015,1:14-15.
[12]	张金花, 王博文, 丁岩 , 等. 转基因大豆种质资源对大豆疫霉根腐病的抗性评价[J]. 东北农业科学, 2019(4):43-49.
[13]	王光飞, 马艳, 郭德杰 , 等. 生物质炭介导生防微生物抑制辣椒疫霉的作用[J]. 中国生态农业学报, 2019(7):1015-1023.
[14]	段春芳, 李月仙, 刘倩 , 等. 32份木薯种质对疫霉根腐病的抗性评价和农艺性状分析[J]. 植物保护, 2017(1):148-152.
[15]	Mohamed Maizatul-Suriza, Matthew Dickinson, Abu Seman Idris , et al. Molecular characterization of Phytophthora palmivora responsible for bud rot disease of oil palm in Colombia[J]. World Journal of Microbiology and Biotechnology, 2019,10:1-23.
[16]	Hao Wel, Gray Morgan A, Forster Helga , et al. Evaluation of new oomycota fungicides for management of phytophthora root of citrus in Colifornia[J]. Plant disease, 2019,4:619-628.
[17]	Khompatara Khemmikar, Pettongkhao Sittiporn, Kuyyogsuy Arnannit , et al. Enhanced resistance to leaf fall disease caused by Phytophthora palmivora in rubber tree seedling by sargassum polycystum extract[J]. Plants, 2019,8(6):168.
[18]	Daniele Palmieri, Edgar Portillo, Yoelvis Sulbarán , et al. Biocontrol of phytophthora root and stem rot disease in papaya (Carica papaya) plants by photorhabdus, the symbiont bacterium of heterorhabditis amazonensis[J]. Biology Control, 2019,10:595-604.
[19]	程颖慧, 王颖, 章桂明 . 泰国榴莲上棕榈疫霉的分离和鉴定[J]. 植物检疫, 2009(3):26-28.
[20]	李雯雯 . 森林疫霉的分类鉴定和遗传多样性研究[D]. 北京:中国林业科学研究院, 2018: 83.
[21]	孙志宁, 崔海辰, 王海丽 , 等. 致病疫霉产孢子囊培养基及诱导方法的筛选[J]. 科学技术与工程, 2017(19):18-23.
[22]	陈亮, 孟玲 . 疫霉病害的发生与化学防治研究进展[J]. 农药, 2013(4):13-16.
[23]	鲍本颜 . 大豆疫霉根腐病的识别与防治[J]. 农技服务, 2011(6):814-837.
[24]	冷冰雪 . 安徽宿州地区大豆疫霉的分离与鉴定[D]. 合肥:安徽农业大学, 2015(6):36.
[25]	谢宁, 曹支敏, 梁超琼 . 花椒疫霉病菌种的鉴定[J]. 西北林学院学报, 2013(2):125-130.
[26]	Noriyuki Miyake, Hirofumi Nagal . Pathogenicity of Phytophthora palmivora to fig fruits after its long-term storage in water[J]. Joumal of General Plant Pathology, 2019,6:251-256.
[27]	Zhang Can, Zhang Wen, Ma Huiqin , et al. First report of phytophthora causing fruit rot of fig (Ficus carica) in China[J]. Plant disease, 2013,97(9):1252.
[28]	冯岩, 陈健, 罗金棠 . 番木瓜棕榈疫霉菌的生物学特性研究[J]. 广东农业科学, 2010(8):136-138.
[29]	王同勇, 杨鹤, 薛玉平 , 等. 7个无花果品种在威海的引种表现及栽培技术[J]. 中国南方果树, 2016(2):161-166.
[30]	Guo Han, Li Chaoping, Shi Tao , et al. First report of Phytophthora palmivora causing root rot of cassava in China[J]. Plant disease, 2012,96(7):1072.
[31]	Athakorn Promwee, Punnawich Yenjit, Montree Issarakraisila , et al. Efficacy of indigenous Trichoderma harzianum in controlling phytophthora leaf fall (Phytophthora palmivora) in Thai rubber trees[J]. Journal of Plant Diseases and Protection, 2017,2:41-45
[32]	Gumtow Rebecca, Wu Dongliang, Uchida Janice . A phytophthora palmivora extracellular cystatin-like protease inhibitor targets papain to contribute to virulence on papaya[J]. Molecular plant-microbe interactions, 2018,3:363-373.
[33]	李琳, 陈鸿宇, 柳凤 , 等. 马拉巴栗疫病病原的分离与鉴定, 园艺学报, 2011,12:2395-2400.
[34]	Wael Alsultan, Ganesan Vadamalal, Ahmad Khairulmazml , et al. Isolation,identification and characterization of endophytic bacteria antagonistic to Phytophthora palmivora causing black pod of cocoa in Malaysia[J]. European Journal of Plant Pathology, 2019,4:134-135.
[35]	Lu Xinhua, Sun Dequan, Rookes James E , et al. Nanoapplication of a resistance inducer to reduce phytophthora disease in pineapple (Ananas comosus L.)[J]. Frontiers in plant science, 2019,10:1238.