Abstract: To evaluate the application perspective of Blumea balsamifera (L.) DC. on defense of agricultural disease, methods including inhibition of mycelia growth and chemical constituents pre-test were adopted to study its anti pathogenic fungi active components. Three fractions including PE (petroleum ether extract), AE (acetic ether extract) and WE (water extract) afforded according to methods of methanol extraction, water suspension and distribution between water and different organic solvents. Concentrations of test samples were prepared as 1, 0.5, 0.25 and 0.125 mg/mL, individually. Results show that the PE part exhibits potent inhibitory activities against growth of two plant pathogenic fungi, namely Alternaria solani (ACCC 36023) and Fusarium gramineum (ACCC 36249), with inhibitory rates of 71%, respectively, at a concentration of 1 mg/mL. The AE part also shows inhibitory activities against Alternaria solani (ACCC 36023) and Fusarium gramineum (ACCC 36249), with inhibitory rates of 58% and 69%, respectively, at a concentration of 1 mg/mL. Accompanied with thin-layer chromatography chemical pre-test, the main antifungal components of PE were postulated to be volatile oils and terpenes, and the AE part mainly contains flavonoids. This study exhibits the PE and AE parts of Blumea balsamifera (L.) DC. contain abundant anti pathogenic active constituents including terpenes and flavonoids, which indicating great value to exploit Blumea balsamifera (L.) DC. as a potential botanical fungicide.