Effect of ABA on Seed-setting Rate and Physiological Characteristics of Rice Leaves Under Low Temperature Stress at Booting Stage

doi:10.11924/j.issn.1000-6850.casb16070120

Abstract

Abstract: The aim is to clarify the regulating effects of ABA under low temperature stress on seed-setting rate and physiological characteristics of rice leaves at booting stage. This study was conducted with two rice cultivars ‘Longjing11’ (cold sensitive) and ‘Longdao5’ (cold tolerant) which were planted in pots. At booting stage, the low temperature treatments (15℃, lasting 1, 2, 3, 4, 5 d) were carried out in climatic laboratory. The different concentrations of abscisic acid (ABA) were sprayed. The regulating effects of ABA on the seed-setting rate of different rice cultivars were studied. The effects of ABA and low temperature on the membrane permeability of leaf and antioxidant enzyme were also discussed. The results showed that the seed-setting rate of ‘Longdao5’ had little change in low temperature condition, and the seed-setting rate of ‘Longjing11’ decreased significantly. Spraying of 20 mg/L ABA could effectively alleviate the cold damage, and the seed-setting rate was improved relatively under low temperature. The activities of SOD, POD and CAT in leaves first showed an increasing trend then a decreasing trend. The activities of antioxidase, contents of soluble sugar and soluble protein of ‘Longdao5’ were higher than those of ‘Longjing11’, but the content of malondialdehyde (MDA) and relative conductivity were lower than that of ‘Longjing11’. It indicated that ABA could increase the cold tolerance of cold sensitive rice cultivar through regulating some physiological characteristics such as the activities of antioxidant enzyme, the content of soluble sugar in leaf.

Xiang Hongtao,Wang Tongtong,Zheng Dianfeng,Wang Lizhi,Luo Yu and Li Wan. Effect of ABA on Seed-setting Rate and Physiological Characteristics of Rice Leaves Under Low Temperature Stress at Booting Stage[J]. Chinese Agricultural Science Bulletin, 2016, 32(36): 16-23.

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