Abstract: To study the optimal nitrogen application rate and period for synergistic improvement of yield and quality of glutinous sorghum, make reasonable cultivation measures and provide theoretical basis for high yield and quality, main varieties in southwest region including‘Lumuo 8’and‘Luzhou Red 1’were used as experimental material, three application levels of nitrogen (N 75, 120, 165 kg/hm2, P 75 kg/hm2 and K 112.5 kg/hm2) and four nitrogen application periods (turning green fertilizer: jointing fertilizer: panicle fertilizer of 0.2:0.2:0.6, 0.2:0.6:0.2, 0.3:0.7:0 and 0.6:0.2:0.2) were designed to study the effect of nitrogen application rate and period on yield and quality of winemaking glutinous sorghum in field conditions. The results showed that in the range of nitrogen fertilization 75-120 kg/hm2, grain yield increased significantly with the increase of N application rate, the increase was not significant when N application rate was more than 120 kg/hm2. The yield of the two varieties reached the highest with the treatment of 0.2:0.6:0.2,‘Lumuo 8’was 8005.5 kg/hm2 and‘Luzhou Red 1’was 6421.1 kg/hm2, but the difference was not significant, the crude protein content increased significantly with the increase of N application rate. While crude starch first increased then decreased with the increase of N application rate, and reached the highest at 120 kg/hm2 N application rate. Nitrogen application period and tannin content had highly significant difference, but it was not significant to the other 4 quality indexes. Under this experiment condition, the optimal N application rate was 120 kg/hm2 and application period for the green fertilizer: jointing fertilizer: panicle fertilizer was 0.2:0.6:0.2.