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Chinese Agricultural Science Bulletin ›› 2017, Vol. 33 ›› Issue (35): 50-56.doi: 10.11924/j.issn.1000-6850.casb16100119

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Screening and Microbial Component of a Thermophilic, Cellulose-degradation Bacterial Community PN-8

  

  • Received:2016-10-29 Revised:2017-01-10 Accepted:2017-01-13 Online:2017-12-14 Published:2017-12-14

Abstract: The aim of this study was to isolate the thermophilic bacterial community with cellulose degradation capacity, and then to solve the rapid degradation problem of cellulose during the composting process. A cellulose-degradation bacterial community, named PN-8 was constructed by the continuous selective culture, and the characteristics and composition of PN-8 was also investigated and analyzed in this study. During the degradation process of rice straw, the pH value of PN-8 suspension firstly dropped then ascended, and the optimum degradation temperature for PN-8 was 55 ℃ with higher degradation ratio up to 80.9%. Through streak plate approach, five bacteria strains were isolated from PN-8. According to the sequence identification, four strains belonged to Bacillales namely Ureibacillus suwonensis,Bacillus thermoamylovorans,Ureibacillus thermosphaericus and Bacillus borbori respectively. Other strain belongded to Serratia sp.. The high-throughput sequencing data showed the consistent result that the Bacillaceae was the predominant bacterial strains in PN-8, accounting for 31.83%. The principal functional bacteria in PN-8 were Bacillaceae, and their favourable cellulose degradation capacity at high temperature would potentially improve the speed and quality of compost.