Abstract: Fishery resources enhancement and conservation fee is an important economic lever for adjustment of industrial structure of marine fisheries. The adjustment process of measures for the collection of fishery resources fee was summarized, and the collection standard and related management enlightenment of the common marine fishery resource fee in Shandong Province and Zhejiang Province were analyzed. Based on the related data, we calculated the receivable standard, and found that the actual collection standard was lower than the calculation results. Then we analyzed the challenges for amendment on the collection of marine fishery resource fee, and found that reducing the burden on agricultural producers and adjustment of oil price subsidy policies were the main challenges. Finally, we put forward some suggestions, such as continuing to implement the marine fishery fee system, refining the standard of marine fishery resource fee, reasonably determining the collection standard, improving the fee standard of resource-destructive type fishing and implementing fishery resource tax.