Abstract: Ammopiptanthus monglicus is an evergreen broadleaf shrub in arid and desert region of north China, and it is also an endangered relic shrub containing excellent gene resource for its adaptation in harsh environment. In this study, the seeds of A. monglicus were germinated in the petri dish containing 1/2 MS solid medium. Then these sterile seedlings were transplanted in a new culture bottle which could provide enough growth space. As some of the lateral roots were adhesion on the medium surface, that would lately induce the formation of hairy lateral root which could be used as young explant for callus induction on CDB1(MS+ 1.0 mg/L 2,4-D+ 0.1 mg/L 6-BA, pH 5.8) medium and callus propagation on CDB6 (MS+ 0.2 mg/L IBA+ 0.1 mg/L KT+ 0.5 mg/L GA3, pH 5.8) medium. Meanwhile, the callus on CDB6 medium could also induce adventitious roots. In addition, the A. monglicus showed characteristics of axillary bud growth and the seedlings of A. monglicus growing in RA (1/2MS+ 0.5 mg/L IBA, pH 5.8) medium could induce the formation of adventitious roots. In conclusion, these results provide some information on the potential of asexual reproduction of A. monglicus and its tissue culture, which could be used for protection and utilization of A. monglicus germplasm.