Abstract: The aims of the experiment are to determine the tibial nerve somatosensory evoked potential (SEP) waveforms in beagle dogsand measure the normal values of the latency period and amplitude. Fifteen healthy Beagle dogs were tested under isoflurane inhalation anesthesia. The tibial nerve was stimulated by electricity with electromyography evoked potentiometer. The self-made reference electrode and the recording electrode were respectively placed at the head Fz point and Cz pointto record SEP waveform. The results showed that fifteen Beagle dogs could record clear bidirectional SEP waveforms by stimulating the left and right tibial nerves. The latency period of the waveforms triggered by stimulation of the left tibial nerve was P (21.71±1.978) ms, N (32.61±2.568) ms, P-N (10.90±1.338) ms, and the coefficient of variation respectively was 9.11%, 7.87%, 12.88%. The amplitude was (2.762±1.230) μV and its coefficient of variation was 41.47%. The latency of the waveforms triggered by stimulation of the right tibial nerve were P (21.49±1.985) ms, N (32.55±2.359) ms, P-N (11.06±1.153) ms, and the coefficient of variation respectively was9.24%, 7.25%, 10.24%. The amplitude was (2.634±1.092) μV and its coefficient of variation was 44.55%.There was no significant difference in latency period and amplitude between right and left side (P>0.05). The SEP waveforms, normal values of the latency period and amplitude of each wave were obtained by measuring the tibial nerve SEPs in normal Beagle dogs. This confirms that the stability of the latency period is superior to the stability of the amplitude. These results can provide a reference for the study of SEP in dogs under pathological conditions, and alsofor the clinical application of SEP in the diagnosis of diseases.