Abstract: To assess the effects of different control measures on spider subcommunity, a field evaluation test of spider subcommunity sampling by vacuum- suction machine was conducted in Henghu Farm of Jiangxi Province in 2015. The results showed that the total numbers and species richness of spider subcommunity in efficient pesticide plots and biological pesticide plots were both significantly lower than that of control plots, no significant differences were found in community indices such as species richness, dominance index, Shannon- Wiener diversity index and evenness index; the temporal dynamics of this four indexes had no obvious differences. Meanwhile, family composition and dominant spider families of spider subcommunity were basically identical, except that dominance of Salticidae in efficient pesticide plots and biological pesticide plots were significantly lower than that of control plots, dominant spider species of spider subcommunity were basically identical in different control measures. Except that the dominance of P. pseudoannulata in efficient pesticide plots was significantly lower than that of control plots, there was no significant difference in dominant spider species under different control measures. This study indicates that efficient pesticides and biological pesticides could both significantly reduce the number and species richness in spider subcommunity, but have no significant difference on the spider subcommunity structure. The biological pesticides have less influence on spider subcommunity compared with the efficient pesticides.