Abstract: Based on the CLUE-S model, combined with region total planning and　ecological protection red line, with an aim to predict the land use pattern in 2040 of the Qingpu District, Shanghai. At last, from the angle of minimum cumulative resistance, the ecological resistance was　compared and analyzed. The results are as follows. (1) To verify, the applicability of CLUE-S model in the study area, the accuracy test index of Kappa calculated and its value reached 95%, indicating the CLUE-S has good reliability in the study area. (2) Land use spatial pattern difference is significant, by 2040, Industrial land, traffic land, residential land, urban green land, forest land, cultivated land and other land present a decreasing trend. (3) Under the ecological protection red line constraint scenario, the ecology resistance of east Qingpu decreases, and the western region remain stable. With time, the land use spatial pattern changes tend to be more reasonable. The study conclusions will provide data reference and basic information of decision support for region future land use planning,management and policy-making.