The Distribution, Development and Utilization of Bioactive Components Resources of Russian Melilotus officinalis

doi:10.11924/j.issn.1000-6850.casb16120122

Abstract

Abstract: [Objective] We have focused on the detection and field development test of Melilotus officinalis bioactive ingredients since 2009 in order to find outSthe content and distribution of bioactive ingredients in Russian Melilotus officinalis. [Methods]We use Kjeldahl method and efficient gas chromatography to measure the proteinScontent, automaticSaminoSacidSanalyzer to measure theSkinds and contents of aminoSacid, Gas chromatography - mass spectrometry (GC - MS) and molecular distillation apparatus to measure the fatty acid content, gravimetric method to measure the Saponin and alkaloid contents, high performance liquid chromatograph to measure the coumarin content, the forint reagent colorimetric method to measure the polyphenol content, anthracene copper - sulfuric acid colorimetry to measure the polysaccharide content, and aluminum nitrate-sodium nitrite-sodium hydroxide colorimetric method to measure the flavone content.[Result]This paper shows the bioactive ingredients content and distribution featuresSin Melilotus officinalis’ roots, stems, leaves, seeds, and measures the total production of bioactive ingredients, i.e. 3016.3 kg/hm2. The bioactive ingredients, crude protein, amino acids and fatty acids, are 1400.6 kg/hm2, 112.2 kg/hm2, 1246.8 kg/hm2, respectively. The output of saponins, alkaloids, polyphenols, coumarin are 97.0 kg/hm2, 77.9 kg/hm2, 41.7 kg/hm2, 40.1 kg/hm2,respectively. [Conclusion] After separation, extraction and purification, the aboveScomponents can be used as the natural ingredients to develop the medicine, food, chemical and other biotechnology products. This paper provides a new way for the development and utilization of natural resources of plants.

CLC Number:

林年丰 and . The Distribution, Development and Utilization of Bioactive Components Resources of Russian Melilotus officinalis[J]. Chinese Agricultural Science Bulletin, 2017, 33(27): 43-48.

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