Abstract: In order to deeply understand the stress response and adaptation of sea fish under ship-rocking condition, provide basic data and technical support for design of deep sea aquaculture farming platform, serum biochemical indexes of spotted knifejaw (Oplegnathus punctatus) under different ship rocking periods and duration were studied by using of a mechanical rocking platform. The results showed that (1) under the short time stress condition (5 min), there was no significant difference between rocking period of 10 s/time group and normal group. But for rocking period 5 s/time group, content of serum glucose significantly decreased, content of cortisol and epinephrine significantly increased, contents of muscle glycogen and hepatic glycogen did not change apparently. (2) Under the long time rocking condition (60 min), there was no significant difference between rocking period 10 s/time group and 5 s/time group. But both of them had remarkable difference with normal group, of which content of serum glucose significantly decreased, contents of cortisol and epinephrine significantly increased, contents of muscle glycogen and hepatic glycogen did not change apparently. (3) Water temperature, salinity, pH, dissolved oxygen concentration did not change remarkably because of rocking. According to the test, short time and slight rocking would not have an effect on O. punctatus. However, violent rocking would cause stress response of O. punctatus to some extent.