Abstract: To determine the variation of plastid pigment degradation products in flue-cured tobacco cultivars in different production areas, the plastid degradation products in the 9th-12th tobacco leaves were analyzed by GC/MS with‘K326’,‘Yunyan 87’and‘Xiangyan 3’planted in typical flavor, middle flavor and fragrant tobacco-producing areas, respectively. The results showed that most plastid degradation products detected in tobacco leaves were significantly different among the three cultivars, and the degradation products of lutein and β-carotenoid were higher in‘Yunyan 87’than those in‘K326’and‘Xiangyan 3’. All of the detected plastid pigment degradation products in leaves were significantly different among the three types of tobacco-producing areas, the three aroma components containing megastigmatrienone A, B and D, which were remarkably higher in typical flavor tobacco-producing area than in middle flavor and fragrant tobacco-producing areas, were sequentially decreased from typical flavor obacco- producing area to middle flavor and fragrant tobaccoproducing areas. The difference of plastid pigment degradation products among cultivars and tobacco producing areas reveals the material basis of aroma formation in tobacco leaves from different ecological planting areas, and is important to developing tobacco cultivars with different flavors.