Abstract: To study the effect of different rotation methods on soil microbial biomass and enzyme activity and screen out the optimum rotation method in the north of Yin Mountain, we conducted an experiment consisted of 10 treatments: 6 rotation patterns, 3 continuous cropping and fallowing, and studied the effect of rotation on soil biological character. The results showed that the soil microbial biomass C content of rotation treatment was significantly higher than that of continuous cropping in 2015, and S2, S6 and S4 were better rotation methods, while S2, S3 and S4 were better rotation methods in 2016. S1 and S4 increased soil microbial biomass N content in 2015, while S4 increased soil microbial biomass N content remarkably in 2016 (P ＜0.05). The catalase activity of all treatments in 2016 was lower than that in 2015, and that of S2、S4、S5 were higher in 2015, while that of S3、S4、S5 were higher in 2016; soil urease and sucrase activity of S1, S2 and S4 increased in 2015, and that of S4, S5 were higher in 2016 than that in 2015, urease activity increased by 24.53% and 23.30%, sucrase activity increased by 21.23% and 19.29%. Soil microbial biomass was significantly correlated with urease activity in 2015, but its correlation with soil enzyme activity was not significant in 2016. In conclusion, soil microbial biomass and soil enzyme activity could be increased by the crop rotation of oatsalfalfa intercropping 1 year, alfalfa 1 year and potato 3 years.