Abstract: A field experiment was conducted to study the effect of different proportions of nano-bentonite coated nitrogen fertilizer and N application levels on growth, yield and nitrogen utilization efficiency (NUE) of late rice. The results showed that the yield, 1000 grain weight and grain number per spike of nano-bentonite coated nitrogen fertilizer treatment were significantly higher than those of CK. Compared with CK, the yield of N application treatment increased by 21.53% to 28.01% (P＜0.05). The effect of nano- bentonite coated nitrogen fertilizer on late rice yield was different under different N application levels, the yield was 7178.54, 7220.28 and 7236.95 kg/hm2 under 10% nano-bentonite coated nitrogen fertilizer application with 0%, 20% and 30% N reduction, respectively, which increased by 1.17%, 1.76% and 1.99% compared with that under conventional N treatment (7095.21 kg/hm2), and the difference was not obvious (P＞0.05). By adding 15% nano- bentonite coated nitrogen fertilizer, the rice yield increased by 2.47% with 0% N reduction and decreased by 0.12%- 3.17% with 20%- 30% N reduction (P＞0.05). Compared with the conventional N treatment, the seed setting rate of nano- bentonite coated nitrogen fertilizer treatment was lower, and the difference was not obvious (P＞0.05); under N reduction, SPAD value of nano- bentonite coated nitrogen fertilizer treatment had no significant difference. The nitrogen accumulation amount in grains at mature period was significantly higher than that without N fertilizer treatment, and 15% nano- bentonite coated nitrogen fertilizer treatment with 20% N reduction enabled the highest N accumulation (68.92 kg N/hm2); the nitrogen use efficiency of nano-bentonite coated nitrogen fertilizer (49.3%~67.1%) was 2.8%~20.6% higher than that of conventional nitrogen fertilizer treatment (46.5%). The results suggest that adding 10% nano-bentonite coated nitrogen fertilizer with 20% N reduction had the best effect on rice yield, setting rate and NUE.