Abstract: Field investigation and indoor analysis method were combined to study soil enzyme activities and nutrients of monsoon evergreen broad-leaved forest in different seasons in Naban River Nature Reserve Area, to explore the impact of seasonal change on enzyme activities and soil nutrients content, and the influence of seasonal change on the relationship between enzyme activities and soil fertilities. The results showed that seasonal variation had a significant impact on soil nutrients content and enzyme activities, different nutrient indicators and enzyme activities showed different variability with the change of season and soil depth, and the coefficient of variation ranged from 0.1 to 1.5; seasonal variation had significant effects on the relationship between each index of nutrient and soil enzyme activities, and the relationship changed with the season; the relationship among soil enzymes was less affected by the change of season, and the correlation was not obvious. There was significant or extremely significant correlation between soil fertilities and enzyme activities of monsoon evergreen broad-leaved forest in the reserve area, the direct influence of soil nutrients on enzyme activities had different degrees of change with seasons.