Gene Cloning, Bioinformatics and Expression Analysis of C3H Gene from Boehmeria nivea L.

doi:10.11924/j.issn.1000-6850.casb17030107

Abstract

Abstract: The aim is to provide a basis for verifying the protein function of coumarate 3-hydroxylase gene (C3H) in the later period, and improving the genetic engineering breeding of Boehmeria nivea variety with low content lignin. Based on the information of high-throughput sequencing of Boehmeria nivea, the full-length sequence of C3H was cloned by the RACE technology, and then the bioinformatics analysis was conducted. The expression pattern of C3H was tested respectively by fluorescent quantitative technology.Bn C3H-1 (cDNA sequence of C3H) with 1940 bp in length was obtained (GenBank accession number: KY078743). The analysis of the physical and chemical properties and structure of BnC3H-1 gene encoding protein showed that the gene encoded a hydrophilic protein containing 511 amino acids with a molecular weight of 57.80 kD, it might be localized in the endoplasmic reticulum. According to the sequence of amino acid and structural analysis, BnC3H-1 contained one conserved domain, cytochrome P450 domain. Phylogenetic analysis showed that the gene had high homology with C3H genes of Eucalyptus grandis and Fagopyrum tataricum.

郭清泉. Gene Cloning, Bioinformatics and Expression Analysis of C3H Gene from Boehmeria nivea L.[J]. Chinese Agricultural Science Bulletin, 2017, 33(32): 21-27.

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