Abstract: Yunnan paris polyphylla growth slowly, it is conducted to study the regulation of the endogenous hormones on growth of Yunnan paris polyphylla. 2 years old and 4 years old and 6 years old of Yunnan paris polyphylla with bud root were collected, the liquid chromatography-mass spectrometry method for the determination of IAA and ABA content in different developmental stages of Yunnan paris polyphylla. The change of the content of IAA in different years in Yunnan paris polyphylla rhizome and bud, have reached a high value in the seedling stage, and in the roots, germination and growth period is 4 years old and 6 years old and 2 years old was higher than. The content of ABA in rhizome tip (bud) was highest in different years, while in the middle of rhizome, ABA content was the highest in 2 years, the growth period was the highest in the year of 4 years and the highest in the 6 years. Except for the seedling stage, the ABA content in different developmental stages was significantly different in different years. Auxin binding changes in Yunnan paris polyphylla from fourth or fifth years, growth into Washings; according to daily growth rates, ABA to promote rhizome swelling.