Abstract: To establish an system for large-scale vegetative propagation and efficient genetic transformation of Rhododendron molle, the leaves of Rhododendron molle plantlets in vitrowere used as explants, the effects of basic medium type, leaf cutting mode, leaf position, darkness culture time and different hormone combinations on the adventitious bud regeneration of leaves were studiedr. The results showed that WPM medium was the optimal basic culture medium. The regeneration frequency of adventitious buds was highest in the leaves which were cut perpendicularly in the main veins and the petioles and leaf apexes were removed. The regeneration ability of leaves at middle and upper parts of a plantlet was stronger, and the induction effect of adventitious bud from leaves at the 5th and 6th positions was the best. The initial dark culture was beneficial to regeneration of leaves and the optimal time of dark culture was 10-15 d. The optimum medium for adventitious bud regeneration was WPM+TDZ1.0 mg/L+ IAA 1.0 mg/L and the regeneration frequency was 82.3% and the average number of adventitious bud was 7.2.