Abstract: The paper aims to study the effect of different spray concentration of Cabrio Top on chlorophyll and nutrient content of cotton. The authors dopted different concentrations of Cabrio Top which were sprayed during the initial flowering period and and flowering and boll-setting period of of cotton, and then measured the content of the total nitrogen, total phosphorus, total potassium in whole plant, root, stem and leaf and the relative value of the chlorophyll content in the function leaves. The results showed that: spraying Cabrio Top with proper concentration (450 -750 g/hm2) had significant promoting function on the accumulation of chlorophyll and the content of the total nitrogen, total phosphorus in the root and stem, but it would reduce the total nitrogen content in cotton leaves; the total potassium content in cotton’s root, stem and leaf was not significant by spraying different concentrations of Cabrio Top; the difference between excessive spray (1050 g/hm2) and water treatment was not significant. Spraying a certain concentration of Cabrio Top in the middle and later periods of the cotton growth can promote the accumulation of chlorophyll and the absorption of nitrogen, phosphorus, to achieve the role of pesticides to promote fertilizer absorption.