Expression Analysis of Key Genes of Chlorophyll Synthesis in A Yellow Leaves Mutant of Oryza sativa

doi:10.11924/j.issn.1000-6850.casb17040104

Abstract

Abstract: The aims are to study the relationship between rice yellow leaf mutant gene and chlorophyll synthesis, and the mechanism of mutant genes. In this study, the wild type was taken as the control, the RNA expression of key genes of chlorophyll biosynthesis in stems and leaves of mutants were analyzed by semiquantitative and real-time quantitative PCR. The results showed that there was no significant difference in the expression of 8 genes of stems in the wild type and the mutants. In the leaves, the expression of YGL1 and CHLI in the mutant was significantly up-regulated, about 1.8 times as that in wild type. The expression of CHLD gene in the mutant was about 0.6 times as that in wild type, was significantly down-regulated. The expression of the other 5 genes was not significantly different between the mutants and the wild type. The results indicated that the mutant gene could regulate the expression of YGL1, CHLD and CHLI genes and further affect the synthesis of chlorophyll in leaves. The study laid a foundation for understanding the role of mutant gene in the regulation of chlorophyll synthesis and its functional mechanism.

CLC Number:

Q756

References

[1] 张天泉.水稻新黄绿叶基因YGL9的鉴定和图位克隆:[D].西南大学,2016
[2] 王亚琴.水稻黄绿叶突变体ygl13的鉴定和候选基因分析:[D].西南大学,2016
[3] Beale, S. I.Green genes gleaned[J].Trends in plant science,2005,10(7):309-312
[4] Bollivar, D. W.Recent advances in chlorophyll biosynthesis[J].Photosynthesis research,2006,90(2):173-194
[5] 刘进.三个水稻叶绿素代谢相关基因的鉴定与功能分析:[D].沈阳农业大学,2016
[6] 王丹霞.水稻yl1黄叶突变体的基因克隆与功能分析:[D].中国农业科学院,2015
[7] Apitz, J. et al.GluTR2 complements a hema1 mutant lacking glutamyl-tRNA reductase 1,but is differently regulated at the post-translational level[J].Plant cell physiology,2014,55(3):645-657
[8] Kumar, A. M. Soll, D.Antisense HEMA1 RNA expression inhibits heme and chlorophyll biosynthesis in arabidopsis[J].Plant physiology,2000,122(1):49-56
[9] Liu, W. et al.Identification and fine mapping of a thermo-sensitive chlorophyll deficient mutant in rice (Oryza sativa L.)[J].Planta,2007,226(3):785-795
[10] Adams, N. B. et al.Characterization of the magnesium chelatase from Thermosynechococcus elongatus[J].The Biochemical journal,2014,457(1):163-170
[11] Adams, N. B. Reid, J. D.Nonequilibrium isotope exchange reveals a catalytically significant enzyme-phosphate complexin the ATP hydrolysis pathway of the AAA(+) ATPase magnesium chelatase[J].Biochemistry,2012,51(10):2029-2031
[12] Adams, N. B. Reid, J. D.The allosteric role of the AAA+ domain of ChlD protein from the magnesium chelatase ofsynechocystis species PCC 6803[J].The Journal of biological chemistry,2013,288(40):28727-28732
[13] Adams, N. B. et al.Nanomechanical and Thermophoretic Analyses of the Nucleotide-Dependent Interactions betweenthe AAA(+) Subunits of Magnesium Chelatase[J].Journal of the American Chemical Society,2016,138(20):6591-6597
[14] Biswal, A. K. et al.Light intensity-dependent modulation of chlorophyll b biosynthesis and photosynthesis byoverexpression of chlorophyllide a oxygenase in tobacco[J].Plant physiology,2012,159(1):433-449
[15] Harper, A. L. et al.Chlorophyllide a Oxygenase mRNA and Protein Levels Correlate with the Chlorophyll a/b Ratioin Arabidopsis thaliana[J].Photosynthesis research,2004,79(2):149-159
[16] Reinbothe, C. et al.A role for chlorophyllide a oxygenase in the regulated import and stabilization of light-harvestingchlorophyll a/b proteins[J].Proceedings of the National Academy of Sciences of the United States of America,2006,103(12):4777-4782
[17] Yang, Y. et al.PGL, encoding chlorophyllide a oxygenase 1, impacts leaf senescence and indirectly affects grain yield and qualityin rice[J].Journal of experimental botany,2016,67(5):1297-1310
[18] 吴自明.水稻黄绿叶基因ygl1的图位克隆及功能分析:[D].南京农业大学,2007
[19] 邢才 et al.植物叶绿素突变体及其分子机理的研究进展[J].生物技术通报,2008,05):10-12+27
[20] Ankele, E. et al.In vivo visualization of Mg-protoporphyrin IX, a coordinator of photosynthetic gene expression in the nucleusand the chloroplast[J].The Plant cell,2007,19(6):1964-1979
[21] Kanesaki, Y. et al.Mg-protoporphyrin IX signaling in Cyanidioschyzon merolae: multiple pathways may involvethe retrograde signaling in plant cells[J].Plant signaling behavior,2009,4(12):1190-1192
[22] Zhang, Z. W. et al.Transient accumulation of Mg-protoporphyrin IX regulates expression of PhANGs - New evidence forthe signaling role of tetrapyrroles in mature Arabidopsis plants[J].Journal of plant physiology,2011,168(7):714-721