Abstract: In order to obtain sequence of TLR8 gene and its expression profiling in different tissues of common carp (Cyprinus carpio), we cloned TLR8 and analyzed the mRNA expression profiling of TLR8 by RT-PCR. The results showed that sequence of TLR8 in C. carpio (GenBank accession No. KT886923) was 1349 bp, with an ORF of 1113 bp. In addition, the cloned TLR8 sequence in C. carpio shared 91% and 82% homologywith that in Sinocyclocheilus graham and Danio rerio, respectively, whereas shared relatively low homology with that in mammals such as Homo sapiens and Mus musculus. The expression analysis showed that TLR8 gene was extensively expressed in all tested tissues in C. carpio, with the highest expression level in gut, followed by in spleen(P<0.05). We successfully obtained the sequence of TLR8 in C. carpio, and demonstrated its tissuespecific expression in gut, spleen and skin, providing a basis for further investigation on the roles of TLR8 in immune response of C. carpio.