Abstract: Two years Lycium.barbarum L.‘Ningqi 7’was used as the material and an conventional drip irrigation was used for CK, three fertilization levels (150, 300 and 450 kg/hm2) were designed to study the effect of different fertilization levels on soil nutrients’characteristics and microbial components. The results showed that: the application of earthworm fermentation liquor increased soil organic matter by 37.70%-51.16%; it also increased the content of alkali-hydrolyzable nitrogen, instant phosphorus and instant potassium, improved the propagation of actinomycetes and fungi, enhanced catalase and urease activity, the highest increasing amount of microbial carbon and nitrogen was 69.37% and 58.38%, respectively, and the yield increased by 9.41%- 34.07% . Among the grey correlation between each trait and yield, the highest was urease, followed by actinomycetes, fungus had the least influence on yield. 300 kg/hm2 earthworm fermentation liquor had the best effect, which could increase organic matter content, improve the release of instant nutrients, increase microorganism growth, reproduction and enzyme activity.