Abstract: The paper aims to explore the effect of light on the seeding cultivation and cold resistance in Strelitzia reginae . The influence of different light condition on the leaf cold resistance abilities is studied by the method of physiology biochemistry from the follow physiologic indexes: the semilethal low temperature (LT50), soluble sugar, praline, POD activity, MDA with 18-months-old Strelitzia reginae as material. The results showed that lethal temperature is also gradually reduced in the natural cooling process, from -1.79℃ to -3.32℃ shade in summer and from -0.72℃ to -1.51℃shade all year. Meanwhile, soluble sugar, proline and MDA changed significantly which showed the strongest cold resistance in summer shade. POD activity was relatively stable, low sensitivity to temperature. The cold resistance of S. reginae growing in the different light condition was: shade in summer, Natural light all year, shade all year.