Arginine Kinase (AK) Fragment of Apolygus lucorum (Meyer-Dür): Cloning and Expressionfrom Different Habitats

doi:10.11924/j.issn.1000-6850.casb17060079

Abstract

Abstract: This study aims to investigate the effects of different habitats on the growth of Apolyguslucorum Meyer-Dür, so as to determine from the molecular levelthe suitable habitat of Apolyguslucorum Meyer-Dür in the orchard. This study used special lure to investigate the occurrence rateof Apolyguslucorum Meyer-Dür in jujube orchardor vineyard.The primers were designed according to the conserved sequence, cloned fragments of arginine kinase and detected gene expression by fluorescence quantitative PCR. The results of the lure investigation showed that the occurrence rate of Apolyguslucorum Meyer-Dür in the jujube orchard was always higher than that in the vineyard. There were three peaks in the jujube, while two in the vineyard. The sequencing results indicated that the target sequence contained 783 nucleotides and could encode 261 amino acids. Sequence alignment and phylogenetic tree indicated that AL-AK had a similarity of 85% or more compared with other insects, and was close to that of Hemiptera. The expression of arginine kinase gene in jujube orchard was lower than that in vineyard. The above results show that the jujube habitat is more suitable than the vineyard for the development of Apolyguslucorum Meyer-Dür, whichprovides the theoretical basis for the control of Apolyguslucorum Meyer-Dür.

References

[1] 陆宴辉, 吴孔明. 棉花盲蝽象及其防治[M]. 北京: 金盾出版社,2008: 29-31.
[2] 肖留斌, 王凤良, 陈华, 等. 绿盲蝽中期预测模型的组建与应用[J]. 应用昆虫学报, 2015, 52(3): 623-630.
[3] 张秀梅, 刘小京, 杨艳敏, 等. 绿盲蝽在Bt转基因棉及枣树上的发生规律[J]. 华东昆虫学报, 2005, 14(1): 28-32
[4] 金银利, 封洪强, 张言芳, 等. 河南冬枣和葡萄上绿盲蝽种群的季节性发生规律[J]. 植物保护, 2015, 41 (2): 149-153.
[5] 王振亮, 韩会智, 刘孟军, 等. 枣园绿盲蝽越冬卵的分布及其孵化规律研究[J]. 西北农林科技大学学报: 自然科学版, 2011, 39(6): 148-152.
[6] 矫振彪, 陆宴辉, 吴孔明. 棉田绿盲蝽的空间分布型及其抽样模型[J]. 应用昆虫学报, 2012, 49(3): 605-609.
[7] 张小兵, 王凯, 王猛, 等. 不同施药方式下吡虫啉对棉田绿盲蝽种群动态的影响[J]. 植物保护学报, 2014, 41(1): 93-97.
[8] 肖留斌, 谭永安, 孙洋, 等. 绿盲蝽对寄主转换的适应性及生理响应[J]. 中国农业科学, 2013, 46(23): 4941-4949.
[9] 孙洋, 柏立新, 张永军, 等. 绿盲蝽丝氨酸蛋白酶基因 AlSP4 的克隆及取食不同寄主植物后的表达谱分析[J]. 昆虫学报, 2012, 55 (6): 641-650.
[10] Werr M,Cramer J, Ilg T, 2009. Identification and characterization of two arginine kinases from the parasitic insect Ctenocephalides felis. Insect Biochem. Mol. Biol., 39(9):634-645.
[11] Uda K, Fujimoto N, Akiyama Y, et al. Evolution of the arginine kinase gene family[J]. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 2006, 1(2): 209-218.
[12] LOHMANN K. Uber die aufspaltung der adenylpyrophosphorsaure and arfininephophorsaure in krebmuskulatur[J]. Bioche-mistry Zeitschilt, 1935, 282: 109-123.
[13]Astrofsky K M, Roux M M, Klimpel K R, et al. Isolation of differentially expressed genes from white spot virus (WSV) infected Pacific blue shrimp (Penaeus stylirostris)[J]. Archives of virology, 2002, 147(9): 1799-1812.
[14] Kotlyar S, Weihrauch D, Paulsen R S, et al. Expression of arginine kinase enzymatic activity and mRNA in gills of the euryhaline crabs Carcinus maenas and Callinectes sapidus[J]. Journal of Experimental Biology, 2000, 203(16): 2395-2404.
[15] Serrano L, Halanych K M, Henry R P. Salinity-stimulated changes in expression and activity of two carbonic anhydrase isoforms in the blue crab Callinectes sapidus[J]. Journal of Experimental Biology, 2007, 210(13): 2320-2332.
[16] Pereira C A, Alonso G D, Ivaudi S, et al. Screening of substrate analogs as potential enzyme inhibitors for the arginine kinase of Trypanosoma cruzi[J]. Journal of Eukaryotic Microbiology, 2003, 50(2):132-134.
[17] 武万锋. 石河子地区棉铃虫抗药性监测及 CYP6AE14 和 AK 基因的克隆[D]. 石河子大学, 2010.
[18] 张元臣, 安世恒, 李为争, 等. 烟夜蛾精氨酸激酶基因的克隆及 mRNA 表达分析[J]. 昆虫学报, 2011, 54(7): 754-761.
[19] 孙洋, 柏立新, 张永军, 等. 绿盲蝽丝氨酸蛋白酶基因 AlSP4 的克隆及取食不同寄主植物后的表达谱分析[J]. 昆虫学报, 2012,(6):641-650.
[20] 许新新, 谭瑶, 高希武. 绿盲蝽 P450 基因的克隆及增效醚对 P450 酶活性的抑制作用[J]. 应用昆虫学报, 2012,(2):324-334.
[21] Wu QY, Li F, Zhu WJ, Wang XY, 2007. Cloning expression, purification, and characterization of arginine kinase from Locusta migratoria mauileusis. Comp. Biochem.Physiol. Part B, 148 (4):355-362.
[22] 李耀发, 高占林, 党志红, 等. 不同类型杀虫剂对绿盲蝽室内毒力及田间药效评价[J]. 河北农业科学, 2008,(1):49-50.
[23] 王丽丽, 王洪涛, 刘学卿, 等. 不同颜色粘虫板对葡萄园绿盲蝽的诱集效果 [J]. 果树学报, 2014,(2): 288-291.
[24] 李耀发, 党志红, 高占林, 等. 不同颜色黏板对绿盲蝽的诱杀效果初步研究[A]. 植物保护科技创新与发展——中国植物保护学会 2008 年学术年会论文集, 2008.
[25] 仝亚娟, 吴孔明, 高希武. 三突花蛛对绿盲蝽和苜蓿盲蝽的捕食作用[J]. 中国生物防治学报, 2009,(2):97-101.
[26] 周利娟, 胡美英, 徐汉虹, 等. γ 辐射对昆虫生理影响的研究进展[J]. 核农学报, 2004, 18(1): 77-80.
[27] 蒙世杰,陈新全.黑腹果蝇P转座因子研究I.我国黑腹果蝇的P-M测定及其地理分布[J].遗传学报,1989(2):118-124.
[28] Brown AE,Grossman SH, 2004. The mechanism and modes of inhibition of arginine kinase from the cockroach (Periplaneta americana).Arch. Insect Biochem. Physiol., 57 (4):166-177.
[29] Zhao YY,Yang G,W ang PG,You MS,2008. Phyllotreta striolata (Coleoptera:Chrysomelidae arginine kinase cloning and RNAi-based pest control.Eur. J. Eutomol.105 (5):815-822.